A Phase I Safety and Immunogenicity Trial of UBI HIV Lipopeptide Vaccine Component P3C541b in HIV-1 Seronegative Human Subjects

Inclusion Criteria Patients must have or be: * Healthy * Negative ELISA for HIV. * One or more HLA alleles: * A33, B8, B27, B35, or Bw62. * Negative for Hepatitis B surface antigen. * Normal urine dipstick. * Normal history and physical examination. * Availability for follow-up planned duration of the study (12 months). * Viable EBV line prior to enrollment. Risk behavior: Required: * Lower risk sexual behavior as defined by AVEG. Exclusion Criteria Co-existing Condition: Patients with any of the following symptoms or conditions are excluded: * Medical or psychiatric condition or occupational responsibilities, which preclude subject compliance with the protocol (e.g., recent suicidal ideation or present psychosis). * Active syphilis. NOTE: If the serology is documented to be false positive due to a remote (\> 6 months) treated infection, the volunteer is eligible. * Hepatitis B surface antigenemia. * Active tuberculosis. NOTE: Volunteers with a positive PPD and a normal chest X-Ray showing no evidence of TB and not requiring INH therapy are eligible. Patients with any of the following prior conditions are excluded: * History of immunodeficiency, chronic illness, malignancy, autoimmune disease. * History of cancer, unless there has been surgical excision followed by a sufficient observation period to give a reasonable assurance of a cure. * History of anaphylaxis or history of other serious adverse reactions to vaccines. * History of serious allergic reaction to any substance, requiring hospitalization or emergent medical care. * History of suicide attempts or past psychosis. Prior Medication: Excluded: * History of use of immunosuppressive medication. * Live attenuated vaccines within 60 days of study. NOTE: * Medically indicated subunit or killed vaccines (e.g., influenza, pneumococcal) are not exclusionary but should be given at least 2 weeks away from HIV immunizations. * Use of experimental agents within 30 days prior to study. Prior Treatment: Excluded: * Receipt of blood products or immunoglobulin in the past 6 months. Risk Behavior: Excluded: * Subjects with identifiable higher risk behavior for HIV infection as determined by screening questionnaire designed to identify risk factors for HIV infection. * History of injection drug use within the last 12 months prior to enrollment. * Higher or intermediate risk sexual behavior as defined by AVEG.