Endotoxin and Inflammatory Markers in Healthy Non-Smokers and Current Smokers Including Patients With Chronic Obstructive Pulmonary Disease (COPD)

Inclusion Criteria: * Healthy non-smokers: normal spirometry (forced expiratory volume in 1 second \[FEV1\] more than or equal to 90% predicted) and normal exhaled NO (between 8 and 24 ppb; flow 50 ml/s) * 0: At risk (current or ex-smokers): normal spirometry, with or without chronic symptoms (cough, sputum production) * I-II: Mild-moderate COPD * FEV1 reversibility of \< 15% after inhaled beta2-agonists * FEV1/forced vital capacity (FVC) \< 70% predicted * FEV1 between greater than or equal to 50% and less than 80% * With or without chronic symptoms (cough, sputum production) * Able to comprehend and grant a written informed consent Exclusion Criteria: * Concomitant use or pre-treatment within the last 4 weeks with oral steroids * Respiratory infection within 4 weeks prior to entry into the trial * Females who are pregnant or lactating * History of current or past drug or alcohol abuse