Effects of Phytoestrogen-rich Diets on Bone Turnover in Postmenopausal Women

Inclusion Criteria: 1. Healthy as assessed by the: * health and lifestyle questionnaire * physical examination * results of the pre-study laboratory tests 2. Caucasian women 3. Postmenopausal (≥12 - ≤60 months since last menses), determined by * interview * FSH level ≥ 20 IU/l 4. Body Mass Index (BMI) ≥22 - ≤29 kg/m2 5. Voluntary participation 6. Having given their written informed consent 7. Willing to comply with the study procedures 8. Willing to accept use of all nameless data, including publication and the confidential use and storage of all data 9. Willing to accept the disclosure of the financial benefit of participation in the study to the authorities concerned Exclusion Criteria: 1. Participation in any clinical trial including blood sampling and/or administration of products up to 90 days before Day 01 of this study 2. Participation in any non-invasive clinical trial up to 30 days before Day 01 of this study, including no blood sampling and/or oral, intravenous, inhalatory administration of products 3. Osteoporosis, determined by * Questionnaire (spontaneous bone fractures, use of medication to treat osteoporosis) * DXA scans of the lumbar spine between day -14 and day 1 of the study; exclusion threshold is set at -2z score of BMD 4. Severe scoliosis (curvature of the spine) that could interfere with the ability of the subject to go through the DXA scanning procedure and/or with a correct reading of the DXA scans 5. Having a history of medical or surgical events that may significantly affect the study outcome, including: * surgical menopause (including hysterectomy) * antecedents and high familiar incidence of breast and/or endometrial cancer * gastrointestinal disease (Crohn's, short bowel syndrome, coeliac disease, gastroenteritis episodes the month before the start of the study) * hepatic disease (acute or viral hepatitis, chronic hepatitis) * cardiovascular disease and thrombosis * impaired renal function * severe immune disease * endocrine diseases (hyperthyroidism, hyperparathyroidism, IDDM, NIDDM) 6. Food allergy as reported by the subject (with special emphasis on soy products) and reported allergy for sunscreen products 7. Use of concomitant medication including * Hormonal replacement therapy (during the study and within the last 6 months before day 01 of the study) * Current use of corticosteroids (including Aerosol therapy) or past use for more than 10 days within the last 6 months * Osteoporosis treatment (biphosphonates, SERM's, calcitonin, injectable PTH) * Other medications known to affect bone metabolism (statins). Use of antibiotics will be carefully recorded. 8. Change in smoking habits for the last 2 months 9. Alcohol consumption \> 21 units (drinks)/week 10. Reported unexplained weight loss or gain of \> 5 % of usual body weight in the month prior to the pre-study screening 11. Reported slimming or medically prescribed diet 12. Professional sportswomen (\> 10 hours extensive sports/week) 13. Reported vegan, vegetarian, macrobiotic food intake 14. Regular intake of soy based foods (\>2 servings per week). Participation is possible when the subject is prepared to stop consumption from screening until the end of the study 15. Taking supplements containing isoflavones, in the 3 months prior to enrolment and during the study. Subjects should not start taking calcium and vitamin D supplements during the study. However, subjects who already take calcium and vitamin D supplements should maintain this intake during the study 16. Recent blood or plasma donation (\<1 month prior to the start of the study) 17. Not willing to stop blood or plasma donation during the study 18. TNO Nutrition and Food Research personnel, their partner and their first and second generation relatives 19. Not having a general practitioner 20. Not willing to accept information-transfer concerning participation in the study, or information regarding his/her health. For example, laboratory results, findings at health and lifestyle questionnaire interview, or physical examination and eventual adverse events communicated to and from their general practitioner 21. Mental status incompatible with the proper conduct of the study