Comparative Study of the Protein C Pathway in Septic and Non Septic Patients With Organ Failure

Assistance Publique - Hôpitaux de Paris90 enrolled

Overview

This is a comparative study performed in 3 groups of patients/subjects: 30 severe sepsis patients, 30 non-septic patients with organ failure, 30 healthy subjects. The only intervention is a venous blood sampling at the onset of the disease. The purpose of the study is to compare the PC pathway and expression and inflammatory genes between the 3 groups. The main hypothesis is that systemic inflammatory response and exacerbated coagulation activation are non specific of an infection as a triggering event.

analysis of the PC pathway * PC activity * PS activity * soluble thrombomodulin * soluble EPCR * activated protein C * quantitative flow cytometry on monocytes (thrombomodulin and EPCR) * whole blood mRNA (Tissue factor, thrombomodulin and EPCR) * inflammatory gene expression analysis by MLPA

Study Type

OBSERVATIONAL

Enrollment

Conditions

Severe Sepsis Organ Failure

Eligibility

Sex: ALLMin age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * severe sepsis patients or: * non-septic acute organ failure patients or: * healthy subjects matched for age and sex with severe sepsis patients Exclusion Criteria: * criteria for organ failure lasting for more than 24 hours

Data from ClinicalTrials.gov