Nasal Epithelial Cells/Blood Lymphocyte Markers for Cystic Fibrosis (CF)/CF Pulmonary Exacerbations

Stable CF Patients: Inclusion Criteria: * Male or female \>= 15 years of age * Confirmed diagnosis of CF * Clinically stable with no evidence of acute upper respiratory tract infection or current pulmonary exacerbation within the previous month * Ability to understand and sign a written informed consent and comply with the requirements of the study Exclusion Criteria: * Chronic use of a medication with anti-neutrophil or anti-inflammatory effect (ibuprofen, systemic or inhaled corticosteroids, or other immunosuppressive agents, etc * Oxygen saturation \<92% on room air * Presence of a condition or abnormality that in the opinion of the Investigator would compromise the safety of the subject or the quality of the data CF patients with pulmonary exacerbations: Male of female \>= 15 years of age Confirmed diagnosis of CF Patient meets a modified definition for a pulmonary exacerbation based upon Fuchs criteria which is treated with intravenous antibiotics for any 4 of the following 12 signs or symptoms: * Increased sputum production * New or increased coughing up of blood * Increased cough * Increased dyspnea with exertion * Malaise, fatigue or lethargy * Anorexia or weight loss * Fever * Sinus pain or tenderness * Changes in sinus discharge * New findings on chest examination * Decline in forced expiratory volume in 1 second (FEV1) \> 10% since previous visit * Radiographic changes indicative of pulmonary infection * Ability to understand and sign a written informed consent and comply with the requirements of the study Exclusion criteria for CF patients with pulmonary exacerbation: * Concurrent use a medication with anti-neutrophil or anti-inflammatory effect within the previous 4 weeks * Presence of a condition or abnormality that in the opinion of the investigator would compromise the safety of the subject or the quality of the data. Inclusion Criteria - Asthma patients * Male or female \>= 15 years of age * Physician diagnosed asthma * Clinically stable with no evidence of acute upper or lower respiratory tract infection or current pulmonary exacerbation within the previous month Exclusion Criteria - Asthma patients * Chronic use of a medication with anti-neutrophil or anti-inflammatory effect within the previous 4 weeks * Treated for an asthma exacerbation with the previous 4 weeks * Treated with oral corticosteroids within the previous 4 weeks * Oxygen saturation \<92% on room air * Presence of a condition or abnormality that in the opinion of the investigator would compromise the safety of the subject or the quality of the data. Inclusion Criteria for Healthy Volunteers * Male or female \>= 18 years of age * Free of any chronic medical condition * Clinically stable with no evidence of acute upper or lower respiratory tract infection within the previous month * Ability to understand and sign a written informed consent and comply with the requirements of the study Exclusion Criteria for Healthy Volunteers * Use of a medication with anti-neutrophil or anti-inflammatory effect within the previous 4 months * Presence of any chronic medical condition * Oxygen saturation \<92% on room air * Presence of a condition or abnormality that in the opinion of the Investigator would compromise the safety of the subject or the quality of the data