Comparison of Sirolimus Alone With Sirolimus Plus Tacrolimus in Type 1 Diabetic Recipients of Cultured Islet Cell Grafts

Inclusion Criteria: * Type 1 insulin-dependent diabetic patients * C-peptide \< 0.15 nmol/l (\<0.45 µg/l) 6 min. after glucagon IV (1mg) (glycemia \> 180 mg/dl) * diabetic nephropathy * hypoglycaemic unawareness Exclusion Criteria: * smoker before transplantation * plasma creatinine \> 2 mg/dl * albuminuria \>1000 mg/24 hrs * abnormal liver function * history of thrombosis or pulmonary embolism * history of malignancy, tuberculosis or chronic viral hepatitis * history of any other serious illness which could be relevant for the protocol * HLA antibodies * EBV antibody negative * HIV 1 \& 2 antibody positive