Context: Cholecystokinin (CCK) and neurotensin are stimulated during meal intake by the presence of fat in the small intestine. The sequence of events suggests that fat hydrolysis is crucial for triggering the release. Objectives: The aim of this study was therefore to investigate whether CCK mediated the effect of intraduodenal (ID) fat on neurotensin secretion via CCK-1 receptors.
Setting: Single center study; 34 male volunteers were studied in consecutive, randomized, double blind, crossover studies. Subjects and Methods: CCK and neurotensin release were quantified in: 1) 12 subjects receiving an ID fat infusion with or without 60 mg orlistat, an irreversible inhibitor of gastrointestinal lipases, in comparison to vehicle. 2) 12 subjects receiving ID long chain fatty acids (LCF), ID medium chain fatty acids (MCF) or ID vehicle. 3) 10 subjects receiving ID LCF with and without the CCK-1 receptor antagonist dexloxiglumide (DEXLOX) or ID vehicle plus IV saline (placebo). Hormone concentrations were measured by specific RIA systems.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
SINGLE
Enrollment
34
1. Triglycerides, long chain fatty acids, medium chain fatty acids perfused to the small intestine 2. Orlistat perfused to the small intestine 3. DEXLOX as CCK-1 receptor antagonist
Clinical Research Center, University Hospital
Basel, Switzerland
Neurotensin plasma concentrations
Time frame: Change in plasma cocnentrations over 2-3 hours
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.