Effect of Sitagliptin on Incretin Effect in Patients With Type 2 Diabetes Mellitus

Inclusion Criteria: * Women with T2DM without childbearing potential * Male patients with T2DM using a double-barrier method of contraception * must be able to complete a 1 week wash-out of current anti-diabetic medications (patients on PPARγ must be off for at least 4 weeks) * no medications which may alter gastric motility (i.e. acetaminophen, erythromycin) except for cardiac medication at a stable dose. * Age 30-70 years * HbA1c ≤9% at screening * BMI\<40 kg/m2 * Must have a fasting blood glucose of ≤11.1 mmol/L (200 mg/dL) at screening * Able to provide written informed consent prior to study participation * Able to communicate well with the investigator and comply with the requirements of the study * Able to maintain dietetic restrictions and to perform measurements of blood glucose on a daily basis (fasting and two-hours postprandial). Patients must be informed the investigator if fasting glucose is above 200mg/dl or two hours postprandially blood glucose concentration above 240mg/dl is being measured. Exclusion Criteria: * T1DM, diabetes as a result of pancreatic injury, or secondary forms of diabetes (eg. Cushing, acromegaly) * Females with childbearing potential, breastfeeding and pregnant women * Need for insulin within the previous 3 months * Use of Thiazolidinediones in the previous 4 weeks * Significant concomitant disease or complications of diabetes (i.e. nephropathy, autonomic dysfunction, orthostasis). * Fasting triglycerides \>5.1 mmol/L (\>450 mg/dL) within the past 4 weeks. * Treatment with systemic steroids and thyroid hormone (unstable dosage). * Patients with any history of gastrointestinal surgery, e.g. partial bowel resections, partial gastric resections, etc. * Participation in any clinical investigation within 4 weeks prior to dosing or longer if required by local regulation. * Donation or loss of 400 mL or more of blood within 8 weeks prior to dosing. * Significant illness within the two weeks prior to dosing. * Past medical history of clinically significant ECG abnormalities or a family history of a prolonged QT-interval syndrome. * History of clinically significant drug allergy; history of atopic allergy (asthma, urticaria, eczematous dermatitis). A known hypersensitivity to the study drug or drugs similar to the study drug. * Any surgical or medical condition which might significantly alter the absorption, distribution, metabolism or excretion of drugs or which may jeopardize the subject in case of participation in the study. The investigator should be guided by evidence of any of the following: * history of inflammatory bowel syndrome, gastritis, ulcers, gastrointestinal or rectal bleeding; * history of major gastrointestinal tract surgery such as gastrectomy, gastroenterostomy, or bowel resection; * history or clinical evidence of pancreatic injury or pancreatitis; * history or presence of impaired renal function as indicated by abnormal creatinine or urea val-ues or abnormal urinary constituents (e.g., albuminuria); * evidence of urinary obstruction or difficulty in voiding at screening; * Polymorphonuclears \<1500/µL at inclusion or platelet count \< 100,000/μL at screening and base-line. * History of immunocompromise. * Evidence of liver disease as indicated by abnormal liver function tests such as SGOT, SGPT, GGT, alkaline phosphatase, or serum bilirubin. SGOT, SGPT, GGT and alkaline phosphatase must not exceed twice the upper limit of the normal range, and serum bilirubin should not exceed the value of 27 µmol/L (1.6 mg/dL). * History of drug or alcohol abuse within the 12 months prior to dosing or evidence of such abuse as indicated by the laboratory assays conducted during the screening evaluations.