Effects of Anthocyanin on Skin Health and Markers of Cardiovascular Disease Risk

Inclusion Criteria: * Female * Upper age limit of 70 years * Minimum of 1 year since last menstruation * Not taking Hormone Replacement Therapy (HRT), and not having taken HRT for a minimum of 6 months prior to commencement * Having no significant past or present medical history of: inflammatory conditions (including eczema, asthma, hay fever, IBS, crohn's disease and arthritis), diabetes, hepatic, renal, cardiac, pulmonary, digestive, haematological, neurological, thyroidal or psychiatric disease. * Agreeing to restrict dietary intake of anthocyanin containing foodstuffs and also other foodstuffs considered to be cardio-protective i.e. red wine, total alcohol, oily fish, dark chocolate, tea and coffee. * Agreeing to maintain existing cosmetic and beauty regime, and avoid 'tanning' (either naturally or synthetically) of the specific sites of skin function measurement during the study. * BMI 20-32 kg/m2 * Successful biochemical, haematological and urinalysis assessment Exclusion Criteria: * allergy / sensitivity to local anaesthetic i.e. Xylocaïne * Regular use of aspirin, or other anti-inflammatory pain relief medication, as prescribed by a doctor. \[If subjects are regular self prescribed aspirin users and are willing to give up aspirin 2 months prior to the study and for the duration of the study they can be included.\] * Taking other counter indicative medication e.g. steroidal medication during the trial, or within 1 month of the trial beginning. * Taking food / dietary supplements e.g. vitamins, minerals, fish oils, plant oils, isoflavones, soy products, carotenoids during the trial (unless prepared to cease intake during, and 1 month preceding the trial). * Those on therapeutic diets or following weight-loss diet. * Current smoker, or smoker in the past year * Having vaccinations or antibiotics within 3 months of start of trial, and those with vaccinations scheduled for during the trial * Parallel participation in another research project which involves dietary intervention and/or sampling of biological fluids/material * Participation in another research project within the last four months unless the total amount of combined blood from both studies will not exceed 470ml. * Those intending to travel during the study to locations where a change in skin colour may be anticipated * Subjects intending to deliberately "sunbathe", use tanning solariums or tanning creams on body sites used for assessment. * Assessed from the clinical screening * abnormal renal function (Na \>145mmol/L, K \>5.0mmol/L, Urea \>7.1mmol/L, Albumin \>50g/L, Creatinine \>125μmol/L, Total bilirubin \>22μmol/L), or liver function (ALP \>126 IU/L, ALT \>50 IU/L), lipid profile (Total cholesterol \> 6.5 mmol/l, TAG \>3.0 mmol/l, HDL\>2.0 mmol/l), anaemia (Haemoglobin \<11.5g/dL), fasting glucose (\> 6.1 mmol/l) or measurements considered to be counter indicative of the trial outcome measures. * Untreated hypertension (\>160/90mmHg) or hypotension (90/50mmHg or 95/50mmHg if symptomatic) * BMI \<19.5 or \>32.5