Thymus Transplantation Dose in DiGeorge #932

Thymus Transplant Inclusion Criteria: * A parent or guardian of the DGS subject signed the consent form. * Medical screening was completed. * For a diagnosis of DGS, the subject had to have one of the following: * Congenital heart disease; * Hypocalcemia requiring replacement; * 22q11.2 hemizygosity or 10p13 hemizygosity; * CHARGE association or CHD7 mutation; * A subject with abnormal ears whose mother had diabetes (type I, type II, or gestational). * To meet the criteria of typical complete DiGeorge Anomaly (cDGA), the subject had to have either: * Circulating CD3+ T cell count by flow cytometry \< 50/mm3 OR * Circulating CD3+ T cells that were also positive for Cluster of Differentiation 45RA (CD45RA)+ CD62L+ and were \< 50/mm3 or less than 5% of total T cells. Thymus Transplant Exclusion Criteria: * Had heart surgery less than 4 weeks prior to projected implant date; * Heart surgery anticipated within 3 months after the proposed time of implantation; * Present or past lymphadenopathy; * Rash associated with T cell infiltration of the dermis and epidermis; * Rejection by the surgeon or anesthesiologist as surgical candidate; * Lack of sufficient muscle tissue to accept a transplant of 4 g/m2 body surface area (BSA) or 0.2 g/kg subject bodyweight; * Had human immunodeficiency virus (HIV) infection; * Had prior attempts at immune reconstitution, such as bone marrow transplant or previous thymus transplantation; * Ventilator support or positive pressure support: Subjects had to be off ventilator or other pressure support such as continuous positive airway pressure (CPAP) or bi-level positive airway pressure (BiPAP) support for 2 weeks prior to enrollment. If the subject was enrolled and was placed back on ventilator or pressure support, the subject had to be able to be weaned off and remain off ventilator or pressure support for 2 weeks. If the subject could not be successfully weaned off ventilator or pressure support, the subject was to be withdrawn from the study. Additional Inclusion Criteria for Parathyroid Transplant Recipient: * 2 tests in patient showing: intact parathyroid hormone (PTH) \< 5 pg/ml when ionized calcium \< 1.1 mmol/L * All inclusion criteria for thymus transplant must be met * 2 involved parents Exclusion for Parathyroid Transplant Recipient: * Parents do not meet enrollment criteria. * Parent(s) decline to be parathyroid donor(s). Parental Parathyroid Donor Inclusion: * \> 18 years old * Answers all questionnaire items and meets safety screening criteria * Normal serum calcium * Normal PTH function * HLA typing consistent with parentage * Parent chosen for donation will share HLA-DR allele in thymus donor; if not applicable, then either parent will be selected (if meet all other criteria). * Must not be on anticoagulation or can come off for donation/transplantation Parental Parathyroid Donor Exclusion: * Donor is only living involved parent or caretaker of the recipient * Hypoparathyroidism - low parathyroid hormone (PTH) in presence of low serum calcium and high serum phosphate * Hyperparathyroidism (or history of) - elevated PTH in presence of high serum calcium and low serum phosphate * History of cancer * Evidence of any of following: HIV-1, HIV-2, HTLV-1, HTLV-2, syphilis, hepatitis B, hepatitis C, West Nile virus, or Trypanosoma Cruzi (Chagas disease) * Elevated AST, ALT, alkaline phosphatase \> 3 times upper limit of normal * History including receipt of a xenograft or risk factors for SARS, Mad Cow - Disease or smallpox. Note: if parent has Mad Cow Disease risk factors (but not active disease), parent(s) may give permission for transplantation. * CMV positive urine * Positive CMV IgM antibodies * Positive IgM anti-EBV VCA * On blood thinners and cannot stop for the parathyroid donation * Elevated PT or PTT (\> ULN) * Platelets \< 100,000 * Positive Toxoplasma IgM * The donor will receive a history and physical; may be excluded based on PI's medical judgment * Hemoglobin \< 9 g/dl * Infectious lesion on head or neck * Goiter on ultrasound * Abnormal fiberoptic laryngoscopy of vocal cords * Pregnancy * Positive HSV IgG is not an exclusion; however, post transplantation prophylaxis is needed * Positive VZV IgG is not an exclusion; however, post transplantation prophylaxis is needed * Medical concern of otolaryngologist * Concern by medical psychologist or social worker. Parents are interviewed together and separately regarding following areas: medical history; health habits; substance use; relationships and support; education/work history; mental status/psychological history; readiness for donation. * Questionnaire (safety screening) responses can lead to exclusion. Biological Mother of DiGeorge Subject Inclusion Criteria: * Competent to provide consent * Willing to provide blood for testing (No other inclusion/exclusion for mother)