Changes of Biomarkers in Response to Training and Antioxidant Treatment

University of Leipzig40 enrolled

Overview

The aim of the study is to compare the effects of an intensive 4 weeks exercise training program with or without additional treatment with antioxidants. 16 volunteers are included into the study and randomized into a treatment (n=8,Vitamine E 400 IU od + vitamine C 1g twice daily) and a non-treatment (n=8, training only) group. Before and after the training program, skeletal muscle biopsies will be taken to measure PGC-1alpha and PPARgamma mRNA expression. In addition before and after the training program individuals will have the following measurements: body weight, waist circumference, body fat content (DEXA scan), euglycemic-hyperinsulinemic clamps, ergospirometer (VO2 max), routine laboratory measurements, serum malondialdehyde concentrations.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

TRIPLE

Enrollment

Conditions

Obesity Type 2 Diabetes

Interventions

Eligibility

Sex: MALEMin age: 25 YearsMax age: 35 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Must be able to swallow tablets * signed informed consent * no concommitant medication * male * age between 25 - 35 years Exclusion Criteria: * drug, tobacco or alcohol abuse * any chronic disease * any acute or chronic inflammatory disease as determined by a leucocyte count \> 7000 Gpt/l, C-reactive protein (CrP) \> 5.0 mg/dl or clinical signs of infection * systolic blood pressure (SBP) \> 140mmHg and diastolic blood pressure (DBP) was \> 85mmHg * cardiovascular or peripheral artery disease * thyroid dysfunction

Outcomes

Primary Outcomes

PGC-1alpha mRNA expression in skeletal muscle PPARgamma mRNA expression in skeletal muscle Serum malondialdehyde concentration

Time frame: after 4 weeks

Secondary Outcomes

body weight glucose infusion rate during the steady state of an euglycemic-hyperinsulinemic clamp VO2max

Time frame: 4 weeks