Allogeneic Mesenchymal Stem Cell for Graft-Versus-Host Disease Treatment

Inclusion criteria: 1\. Informed consent. 2. Any patient that has undergone allogeneic stem cell transplantation with steroid refractory grades II-IV acute GvHD either occurring post transplant, or induced by donor lymphocyte infusions (DLI) or T-cell add back. A positive biopsy for GvHD is not required if clinical signs and symptoms are characteristic for GvHD and other etiologies are excluded. 3\. Patient received best known therapy for GvHD including: i. Patients must receive cyclosporine A (trough level 150-300 ng/ml) or tacrolimus (trough level 5-15 ng/ml). ii. In addition, steroids must have been given, for instance prednisolone ≥2 mg/kg/day (or equivalent doses of methylprednisolone, etc.) for at least 72h in case of progressive acute GvHD, 5 days non progressive acute GvHD. iii. Despite this treatment, the patient has unresponsive GvHD after 5 days or progressive acute GvHD after 72 hours. If single organ acute GvHD grade II from gut or liver, either progression from single organ or addition of one or two more organs; e.g., if the patient has grade II acute GvHD of the skin, GvHD is more intense and more widespread, or GvHD also includes liver and/or gut. iv. Patients with steroid refractory GvHD fulfilling the requirements mentioned in a) - c) may be treated with second line therapy, e.g., MMF, serotherapy, ECP, change of CsA for tacrolimus or vice versa, etc. Failure to respond to additional treatment similar to what is described for steroids in c) is necessary before enrolment in this study. v. Termination of all GvHD medications other than cyclosporine/tacrolimus/MMF and prednisolone is strongly encouraged. Exclusion criteria 1. Patients with poor performance, not expected to survive 5 days. 2. Patients with a history of hypersensitivity to penicillin and/or gentamycine 3. Poor compliance. Donor inclusion criteria: 1. MSC donor must be HIV, HB-s antigen, anti HBc and anti HCV negative. 2. First choice original HSC donor HLA-identical sibling donor. 3. Second choice mismatched related or unrelated donor (for instance MSC frozen and left over from another patient). 4. Third choice or emergency pre-expanded third party umbilical cord/placenta derived MSC. Donor exclusion criteria: 1. Donor more than 65 years of age, or unhealthy. 2. Donor who is positive for HIV, hepatitis Bs antigen, HB-s, anti-HBc and anti HCV negative.