Collection of Samples for the Evaluation of Wound Biofilm in Acute and Chronic Wounds

Inclusion Criteria: * The subject must be 18 years of age or older. * The subject must have a full thickness acute or chronic wound. The sampling is restricted to subjects with the presence of a full thickness wound in order to reduce any risk for infection to the subject. Biopsying a wound that is already full thickness should not increase the subject's risk. * The subject must have no history of coagulopathy * If the subject's acute wound will be biopsied, the wound must be less than 48 hours old. * The subject must be a candidate for sharp debridement Exclusion Criteria: * The subject may not have myelosuppression indicated by a CBC which may include a WBC less than 1500 cells per cubic millimeter, platelet count less than 125,000 per cubic millimeter, or hemoglobin less than 9 g/dL. * The subject may not be on anticoagulation therapy because anticoagulation therapy increases the risk of bleeding. * The subject may not have a TCpO2 less than 20 torr. Subjects with low tissue oxygenations may have some risk from biopsy due to their poor ability to heal and, therefore, are excluded. * The subject may not be pregnant * The subject's wound must not be in the region of the face, neck, or genitalia