Bioequivalence Study of Hydrochlorothiazide 50mg Tablets Under Fasting Conditions

Inclusion Criteria: * Aged 18-45 years. * Were neither overweight nor underweight for his height as per the Life Insurance Corporation of India height/weight chart for non-medical cases. * Had voluntarily given written informed consent to participate in this study. * Were of normal health as determined by medical history and physical examination of the subjects performed within 21 days prior to the commencement of the study. Exclusion Criteria: * \- Had history of allergy or hypersensitivity to hydrochlorothiazide or any other sulphonamide derived drugs. * Had history of diarrhoea, vomiting or headache within past two weeks * Had history of hypotension (systolic BP\<100 mmHg) * Had history of allergy or bronchial asthma * Had history of muscle cramps or muscle weakness * Had history of any anaphylactic reactions, necrotizing angiitis (vasculitis and cutaneous vasculitis), respiratory distress including pneumonitis and pulmonary edema, photosensitivity, fever, urticaria, rash, purpura, Systemic Lupus Erythematoses(SLE) * Had history of aplastic anemia, agranulocytosis, leukopenia, hemolytic anemia, thrombocytopenia * Had history of anuria or history/evidence of any renal disorder including renal failure, renal dysfunction, interstitial nephritis. * Had history of erythema multiforme including Stevens- Johnson syndrome or exfoliative dermatitis including toxic epidermal necrolysis * Had history of pancreatitis * Had history/evidence of jaundice or any hepatic or gall bladder disease * Had history of hyperglycemia, glycosuria, hyperuricemia or gout. * The subject had a history/evidence of any electrolyte imbalance, serum sodium \< 135 mEq/L, serum potassium \< 3.5 mEq/ L. * Had any evidence of organ dysfunction or any clinically significant deviation from the normal, in physical or clinical determinations. * Had presence of disease markers of HIV 1 or 2, Hepatitis B or C viruses or syphilis infection. * Had presence of values, which are significantly different from normal, reference and/or judged clinically significant for haemoglobin, total white blood cells count, differential WBC count or platelet count. * Had positive for urinary screen testing of drugs of abuse (opiates or cannabinoids) * Had presence of values, which are significantly different from normal, reference ranges and/or judged clinically significant for serum creatinine, blood urea nitrogen, serum aspartate aminotransferase (AST), serum alanine aminotransferase (ALT), serum alkaline phosphatase, serum bilirubin, plasma glucose or serum cholesterol. * Had clinically abnormal chemical and microscopic examination of urine defined as presence of RBC, WBC (\>4/HPF), epithelial cells (\>4/HPF), glucose (positive) or protein (positive). * Had clinically abnormal ECG or Chest X-ray. * Had history of serious gastrointestinal, hepatic, renal, cardiovascular, pulmonary, neurological or haematological disease, diabetes or glaucoma. * Had history of any psychiatric illness, which may impair the ability to provide, written informed consent. * Were regular smokers who smoke more than 10 cigarettes daily or have difficulty abstaining from smoking for the duration of each study period. * Had history of drug dependence or excessive alcohol intake on a habitual basis of more than 2 units of alcoholic beverages per day (1 unit equivalent to half pint of beer or 1 glass of wine or 1 measure of spirit) or have difficulty in abstaining for the duration of each study period. * Had used any enzyme modifying drugs within 30 days prior to Day 1 of this study. * Had participated in any clinical trial within 12 weeks preceding Day 1 of this study. * Subjects who, through completion of this study, would have donated and/or lost more than 350 mL of blood in the past 3 months.