Fermented Milk Drink on Human Intestinal Microflora

Cheng Hsin Rehabilitation Medical Center24 enrolled

Overview

Fermented milk is a popular drink. Recent studies revealed that some fermented milk containing some strains of lactic acid bacteria have health-promoting effects through improvement of the intestinal microflora and modulation of the immune system. Lactobacillus casei strain Shirota (LcS) is a probiotic bacterium used in the production of fermented milk products and lactic acid bacteria preparations. LcS has been shown to modify allergen-induced immune responses in allergic rhinitis, altering fermentation patterns in the small bowel, prevention of recurrent urinary tract infections in women. Yakult®300 is a fermented milk containing at least 3x10\^10 live LcS cells per 100 ml bottle. This study aimed to evaluate the effects of a fermented milk drink containing Lactobacillus casei strain Shirota on the human intestinal microflora.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

DOUBLE

Enrollment

Conditions

Signs and Symptoms, Digestive

Interventions

Eligibility

Sex: ALLMin age: 20 YearsMax age: 65 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Healthy adult volunteers and willing to give voluntary written informed consent Exclusion Criteria: * Major systemic disease * Pregnancy, parturient and feeding woman, or expect to be pregnant * Abnormal liver function * Abnormal renal function * Abnormal gastrointestinal function * Take medication for gastrointestinal function * Stroke, myocardial infarction, * Poor compliance for study protocol

Outcomes

Primary Outcomes

Modification of the Composition of the Intestinal Microflora: Bifidobacterium

Fecal specimens were obtained from all 24 healthy volunteers at week 0, 1, 2, 3, 4, 5, 6. Bifidobacterium was cultured. Bacterial colonies were counted.

Time frame: week 0, 1, 2, 3, 4, 5, 6.

Modification of the Composition of the Intestinal Microflora: Clostridium Perfringens

Fecal specimens were obtained from all 24 healthy volunteers at week 0, 1, 2, 3, 4, 5, 6. Clostridium perfringens was cultured.Bacterial colonies were counted.

Time frame: week 0, 1, 2, 3, 4, 5, 6.

Secondary Outcomes

Modification of the Composition of the Intestinal Microflora: Escherichia Coli, Lactobacillus Spp., Lactobacillus Casei Shirota

Fecal specimens were obtained from all 24 healthy volunteers at week 0, 1, 2, 3, 4, 5, 6. Bacterial colonies were cultured and counted.

Time frame: at week 0, 1, 2, 3, 4, 5, 6