Medical center personnel were screened organoleptically for bad breath by 2 investigators using Rosenberg scale 0-5, and measurement of breath sample in portable gas chromatograph. With a threshold score of 2 or more, or 75parts per billion(ppb) hydrogen sulfide(H2S), subjects invited to enter clinical trial of the mechanical effect of daily tongue scraping with the adjunctive use of 0.12% chlorhexidine gluconate mouthwash or 0.1% stabilized chlorine dioxide mouthwash. Subjects randomly assigned in double-blind trial for one week. The null hypothesis is there will be no difference between the two rinses as adjuncts to tongue scraping.
Subjects in trial had oral and periodontal exam and scored the Ramfjord teeth(#3, 9, 12, 19, 25, 28) for Plaque Index(PlI), Gingival Index(GI), probing depth (PD), recession. A tongue coating index is scored and sample of coating on dorsum taken for culture of total viable count and percentage of black sulfide-producing colonies on anaerobe agar with lead acetate added. Subjects given hygiene instruction and instruction on use of scraper, then rinse with 20ml assigned mouthwash 30sec. Breath assessed by organoleptic means and mouth air sample concentration of hydrogen sulfide, methyl mercaptan in portable gas chromatograph at 0, 1, 2, and 4 hours. Subjects use scraper and mouthwash twice daily for one week and return for final exam.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
QUADRUPLE
Enrollment
22
20ml of mouthwash used for 30sec as adjunct to tongue scraper twice a day
The intervention was accomplished by subject after instructions by investigator: twice a day the scraper was used for 4 strokes then 20ml 0.12% chlorhexidine gluconate rinse for 30sec, for one week.
UCaliforniaSF
San Francisco, California, United States
Percentage of Participants With Rosenberg Score at Indicated Time Points
2 investigators are trained to evaluate smell using the Rosenberg scale which measures foul smelling breath. The Rosenberg scale is validated and is scored 0-5 with 0= no bad breath, 5=worst bad breath. A score of 2 is the threshold at which bad breath is determined.
Time frame: baseline, 1 hour, 2 hours, 4 hours, 1 week
Concentration of Hydrogen Sulfide (H2S) in Mouth Air at 1 Hour
Using portable gas chromatograph
Time frame: 1hr
Concentration of Hydrogen Sulfide (H2S) in Mouth Air at 2 Hours
Using portable gas chromatograph
Time frame: 2hr
Concentration of Hydrogen Sulfide (H2S) in Mouth Air at 4 Hours
Using portable gas chromatograph
Time frame: 4 hours
Concentration of Hydrogen Sulfide (H2S) in Mouth Air at 1 Week
Using portable gas chromatograph
Time frame: 1 week
Concentration of Methyl Mercaptan (MM) in Mouth Air at 1 Hour
Using portable gas chromatograph
Time frame: 1 hour
Concentration of Methyl Mercaptan (MM) in Mouth Air at 2 Hours
Using portable gas chromatograph
Time frame: 2 hours
Concentration of Methyl Mercaptan (MM) in Mouth Air at 4 Hours
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Using portable gas chromatograph
Time frame: 4 hours
Concentration of Methyl Mercaptan (MM) in Mouth Air at 1 Week
Using portable gas chromatograph
Time frame: 1 week
Number of Bacteria on Tongue at 1 Week
Total viable count(TVC) in colony forming units(CFU) on anaerobe agar
Time frame: 1 week
Percentage of Sulfide-producing Black Colonies Out of Total Viable Count(TVC) on Anaerobe Agar Containing Lead Acetate
Time frame: 1 week