Blood Samples From Patients With Non-Small Cell Lung Cancer and From Healthy Volunteers

University of Minnesota84 enrolled

Overview

RATIONALE: Studying samples of blood from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This research study is looking at blood samples from patients with non-small cell lung cancer and from healthy volunteers.

OBJECTIVES: * Measure plasma cathepsin D levels in patients with non-small cell lung cancer and in healthy volunteers using mass spectometry. * Measure fatty acid synthase levels in these patients and healthy volunteers using mass spectometry. OUTLINE: Blood samples are collected from patients and healthy volunteers to measure the levels of cathepsin D and fatty acid synthase by proteomic profiling and mass spectrometry.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Lung Cancer

Interventions

Mass SpectrometryOTHER

Levels of Cathepsin D (CD) and fatty acid synthase (FAS) will be measured using isobaric tag labeling coupled with mass spectrometry. RNA concentration will be measured using spectrophometer and purity will be analyzed.

Eligibility

Sex: ALLMin age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: Non-Small Cell Lung Cancer Patients * Documented diagnosis of non-small cell lung cancer, any stage and any time point in the course of treatment (i.e., at diagnosis, during any treatment, and during post treatment surveillance) * Measurable disease by CT scan within the past 4 weeks Healthy volunteer * Non-smoking, defined as \< 5 packs/year history of smoking and cessation of smoking ≥ 6 months ago OR a never smoker * Not regularly exposed (i.e., daily) to second-hand smoke Exclusion Criteria: * Severe underlying lung disease (i.e., chronic obstructive pulmonary disease with FEV\_1 \< 1.0 L, pulmonary fibrosis, bronchiectasis, cystic fibrosis, etc.) as determined by enrolling researcher. Written consent must be given for both subjects and controls.

Locations (1)

Masonic Cancer Center at University of Minnesota

Minneapolis, Minnesota, United States

Outcomes

Primary Outcomes

Levels of cathepsin D

Measure plasma CD levels in patients with non-small cell lung cancer and non-smoker healthy controls using mass spectrometry.

Time frame: At Routine Follow-Up

Secondary Outcomes

Levels of fatty acid synthase (FAS)

Measure FAS levels in patients with non-small cell lung cancer and non-smoker healthy controls using mass spectrometry.

Time frame: At Routine Follow-Up

Global microRNA expression

Measure global microRNA expression by microarray analysis using Illumina probes.

Time frame: At Routine Follow-Up

Data from ClinicalTrials.gov

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