DNA Analysis of Tissue Samples From Patients With Head and Neck Cancer

Vanderbilt-Ingram Cancer Center74 enrolled

Overview

RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This laboratory study is looking at DNA in tissue samples from patients with head and neck cancer.

OBJECTIVES: * Assess common genetic mutations in tumor and germline DNA samples from patients with head and neck squamous cell carcinoma (HNSCC). * Search for viral DNAs and relate this information to demographics, tumor characteristics, and patient prognosis. * Search for novel viral sequences and cancer-associated gene mutations in young nonsmoking HNSCC patients and in other distinct head and neck cancers. * Determine tumor suppressor gene mutations in HNSCC patients and relate this information to gene expression data. * Validate gene expression information using independent techniques such as real-time PCR on expressed sequences and immunohistochemistry. OUTLINE: Genetic mutation analysis and gene expression studies are performed on tissue samples from patients with head and neck squamous cell carcinoma. Techniques used include real time-PCR and immunohistochemistry.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Head and Neck Cancer

Interventions

cytogenetic analysisGENETIC

gene expression analysisGENETIC

mutation analysisGENETIC

polymerase chain reactionGENETIC

Eligibility

Sex: ALLMin age: 18 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Patient with head and neck squamous cell carcinoma (HNSCC) enrolled in the Head and Neck Tissue Repository * Sufficient biological material collected to perform study tests * Historical material available from excess tissues at the Vanderbilt Pathology Department Exclusion Criteria: * Not specified PRIOR CONCURRENT THERAPY: * Not specified

Outcomes

Primary Outcomes

Assess common genetic mutations in tumor and germline DNA samples

Time frame: Upon collection of final patient data

Viral DNAs related to demographics, tumor characteristics, and patient prognosis

Time frame: Upon collection of final patient data

Novel viral sequences and cancer-associated gene mutations in young nonsmoking patients with head and neck squamous cell carcinoma and in other distinct head and neck cancers

Time frame: Upon collection of final patient data

Tumor suppressor gene mutations related to gene expression data

Time frame: upon collection of final patient data

Validation of gene expression information using independent techniques such as real-time PCR on expressed sequences and immunohistochemistry

Time frame: upon collection of final patient data

Data from ClinicalTrials.gov

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