RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This laboratory study is looking at tissue samples from patients with T-cell acute lymphoblastic leukemia.
OBJECTIVES: * Identify the transcriptional signatures associated with the presence of NOTCH1 mutations in primary T-cell acute lymphoblastic leukemia (T-ALL) cells using oligonucleotide microarrays. * Characterize the global changes on gene expression resulting from the inactivation of NOTCH signaling in human T-ALL lymphoblasts. OUTLINE: This is a multicenter study. Frozen lymphoblast samples from patients with T-cell acute lymphoblastic leukemia (NOTCH1-mutated and wild type) are assessed for genetic expression profiles and mutations by microarray analysis and activated NOTCH1 protein by western blot analysis. PROJECTED ACCRUAL: A total of 48 samples from patients will be accrued for this study.
Study Type
OBSERVATIONAL
Enrollment
49
Transcriptional signatures associated with NOTCH1 mutations in T-cell acute lymphoblastic leukemia (T-ALL) cells
Mutational analysis
Time frame: 1 month
Global changes on gene expression resulting from inactivation of NOTCH signaling in T-ALL cells
Gene expression
Time frame: 1 month
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