Cyclooxygenase-2 Expression in Tissue Samples From Patients With a Normal Cervix, Cervical Intraepithelial Neoplasia, or Early Invasive Cervical Cancer

Wake Forest University Health Sciences

Overview

RATIONALE: Studying samples of tissue from patients with or without cancer in the laboratory may help doctors identify and learn more about biomarkers related to cancer. PURPOSE: This laboratory study is looking at cyclooxygenase-2 expression in tissue samples from patients with a normal cervix, cervical intraepithelial neoplasia, or early invasive cervical cancer.

OBJECTIVES: * Determine cyclooxygenase-2 (COX-2) levels in biopsy or surgical specimens from patients with normal cervical epithelium, cervical intraepithelial neoplasia, or microinvasive squamous cell carcinoma of the cervix. * Correlate COX-2 levels with histological diagnosis and parameters of invasion (i.e., matrix metalloproteinase, microvessel count, and VEGF). OUTLINE: Paraffin-embedded tissue samples are analyzed by immunohistochemistry for evaluation of cyclooxygenase-2 levels, microvessel count, and matrix metalloproteinase and VEGF expression. Medical data for study analysis is obtained by retrospective review of patient charts.

Study Type

OBSERVATIONAL

Conditions

Cervical Cancer Precancerous Condition

Interventions

immunohistochemistry staining methodOTHER

laboratory biomarker analysisOTHER

medical chart reviewOTHER

Eligibility

Sex: FEMALEMin age: 18 YearsMax age: 120 Years

Medical Language ↔ Plain English

DISEASE CHARACTERISTICS: * Meets 1 of the following criteria: * Underwent hysterectomy with removal of a normal cervix for diagnosis unrelated to cancer or cervical dysplasia * Underwent cervical biopsy or removal of cervical tissue and diagnosed with cervical intraepithelial neoplasia (CIN) grades 1-3 * Underwent cervical biopsy and diagnosed with microinvasive squamous cell carcinoma of the cervix * Paraffin-embedded tissue samples of normal cervical epithelium, CIN, or microinvasive squamous cell carcinoma of the cervix must be available for examination PATIENT CHARACTERISTICS: * Not specified PRIOR CONCURRENT THERAPY: * Not specified

Outcomes

Primary Outcomes

Cyclooxygenase-2 (COX-2) levels in cervical intraepithelial neoplasia (CIN)

Time frame: day 1

Correlation of COX-2 levels in CIN with histological diagnosis and parameters of invasion

Time frame: Day 1

Data from ClinicalTrials.gov

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