The purpose of this study is to determine the partitioning of exogenous lipids in the postprandial period while a study of overfeeding. The method is based on the incorporation of a stable isotopic tracer (d31\_palmitic acid, d31\_C16) in lipoprotein triglycerides (TG-CHYLOMICRON and TG-VLDL) and in free fatty acids (FFA).
Healthy overweight and lean young men are subjected to an overfeeding during 56 days which corresponds to a supplement of 761 Kcal/day. During two exploration days (before : D0 et after : D56 overfeeding) they have ingested a breakfast with tracer (d31\_palmitic acid, d31\_C16, 20mg/kg) and blood and urine samples were collected every hour of each exploration day. The enrichment in deuterium was measured by gas chromatography-organic mass spectrometry (GC-OMS) in palmitic acid pool of lipid fractions.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
40
100g per day of cheese + 40g per day of almonds + 20g per day of butter
Centre de recherche en nutrition humaine Rhone-Alpes
Pierre-Bénite, France
Post prandial partitioning of exogenous lipids
Time frame: Before and after overfeeding
Genes expression in adipose and muscle tissues
Time frame: Before and after overfeeding
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