Assessment of Candidate Protein Expression in Breast Cancer Specimens

Case Comprehensive Cancer Center460 enrolled

Overview

RATIONALE: Studying the genes expressed in samples of tumor tissue from patients with cancer may help doctors identify biomarkers related to cancer. PURPOSE: This research study is looking at tumor tissue samples from patients who have undergone surgery for breast cancer.

OBJECTIVES: * To determine if candidate genes display differential expression between normal and cancer breast tissues. OUTLINE: Previously collected breast cancer tissue samples (paraffin embedded blocks) and normal adjacent tissue are used for immunohistochemistry studies of expression of candidate genes.

Study Type

OBSERVATIONAL

Enrollment

460

Conditions

Breast Cancer

Interventions

gene expression analysisGENETIC

Previously collected breast cancer tissue samples (paraffin embedded blocks) and normal adjacent tissue are used for immunohistochemistry studies of expression of candidate genes.

immunohistochemistry staining methodOTHER

Previously collected breast cancer tissue samples (paraffin embedded blocks) and normal adjacent tissue are used for immunohistochemistry studies of expression of candidate genes.

laboratory biomarker analysisOTHER

Previously collected breast cancer tissue samples (paraffin embedded blocks) and normal adjacent tissue are used for immunohistochemistry studies of expression of candidate genes.

Eligibility

Sex: ALL

Medical Language ↔ Plain English

Inclusion Criteria: DISEASE CHARACTERISTICS: * Diagnosis of breast cancer * Tissue samples available * Samples are selected based on presence or absence of tumor receptors, (i.e.; estrogen, progesterone, and HER2 receptors) and grouped into following 4 categories: * Estrogen receptor (ER)-positive/progesterone receptor (PR)-positive/HER2-negative * ER-positive/PR-positive/ HER2-positive by FISH * ER-negative/PR-negative/HER2-positive * ER-negative/PR-negative/HER2-negative(triple negative or basal-type cancers) PATIENT CHARACTERISTICS: * Menopausal status not specified PRIOR CONCURRENT THERAPY: * Not specified Exclusion Criteria: * There are no exclusion criteria in this protocol

Locations (1)

University Hospitals Cleveland Medical Center, Case Comprehensive Cancer Center

Cleveland, Ohio, United States

Outcomes

Primary Outcomes

Differential expression of candidate genes between normal and cancer breast tissues

Approximately 5 slides of each tumor will be collected to provide sufficient samples for replicate staining, if necessary, as well as a staining for a positive control

Time frame: non-applicable

Data from ClinicalTrials.gov

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