Efficacy & Safety of Autologous Dendritic Cell Vaccination in Glioblastoma Multiforme After Complete Surgical Resection

Clinica Universidad de Navarra, Universidad de Navarra26 enrolled

Overview

1. Primary outcome measure: a.Evaluation of the treatment impact on progression-free survival. 2. Secondary outcome measures: 1. Safety evaluation. * Direct effects attributable cell obtaining and administration. * Adverse events during treatment. * Neurological deterioration quantified using the NIH Stroke Scale. * Autoimmune phenomena. 2. Evaluation of impact on other efficiency clinical parameters. * Overall survival. * Quality of life measured with EORTC questionnaire. 3. Study of specific immune response and correlates with clinical outcome. * Delayed hypersensitivity. * Humoral response to autologous tumor cells/tumoral lysate. * Cellular response (proliferation, cytokine production, specific cytotoxicity). 4. Cell line characterization and correlate the final product with clinical efficacy. * Phenotypic studies.

A prospective, open-label, unicentric phase II trial, historical control and non-randomized. The study will try to evaluate the efficiency and safety of the experimental treatment using a cell therapy product (tumor lysate-pulsed autologous dendritic cell vaccine) in patients with glioblastoma multiforme in whom a gross total resection is feasible. Patients will receive standard first-line therapy (surgery before radio-chemotherapy) along with the experimental treatment. The experimental treatment consists in subcutaneous vaccination with a suspension of autologous dendritic cells (cells from the same patient) produced by cell culture from monocytes from the same patient extracted by leukapheresis and pulsed with a lysate of the patient´s tumoral tissue. The first four vaccines will be administered on a monthly basis, concomitantly with the standard chemo and radiotherapy treatments, the next four vaccines, every other month and the four last vaccinations every three months.The results obtained will be compared with those of an historical control study, where patients received a standard treatment without the experimental vaccine.

Study Type

INTERVENTIONAL

Allocation

Purpose

TREATMENT

Masking

Interventions

autologous dendritic cellsBIOLOGICAL

Patients will receive standard first-line therapy (surgery before radio-chemotherapy) along with the experimental treatment. The experimental treatment consists in subcutaneous vaccination with a suspension of autologous dendritic cells (cells from the same patient) produced by cell culture from monocytes from the same patient extracted by leukapheresis and pulsed with a lysate of the patient´s tumoral tissue. The first four vaccines will be administered on a monthly basis, concomitantly with the standard chemo and radiotherapy treatments, the next four vaccines, every other month and the four last vaccinations every three months.The results obtained will be compared with those of an historical control study, where patients received a standard treatment without the experimental vaccine.

Eligibility

Sex: ALLMin age: 18 YearsMax age: 70 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Patients with histological diagnosis of glioblastoma that have not received any previous chemotherapy or radiotherapy treatment. * Patients are able to give informed consent and willing to comply with the protocol requirements during the study period. * Age between 18 and 70 years * Negative pregnancy test In female fertile subjects * Patient is, in the investigator's opinion, willing and able to comply with the protocol requirements. * Complete/Total resection of tumour with surgery guided by fluorescence microscopy and 5-aminolevulinic acid, observed with post operative magnetic resonance imaging. The residual lesion must be null or ≤ 1 cm3 by contrast capturing. * Enough tumor tissue available for the cellular vaccine elaboration Exclusion Criteria: * Patients with infections, severe diseases or hepatic, renal or medullary failures, that in the investigator's opinion, are not eligible to participate in the study. * Participation in other clinical trial. If the patient has participated in other clinical trial within previous months, the patient has to complete the washout period required by de the investigator. * Patients with diagnosis of other neoplasia, except basal cell or squamous cell skin, carcinoma in situ of the cervix properly treated or other tumour curatively treated and no evidence of relapse for at least 3 years. Those cases with coexisting tumours of long-term survival prediction will be considered individually. * Pregnant or breast-feeding women. * Patients who need immunosuppressive drugs. * Positive serology for HIV , hepatitis B (HBsAg) or hepatitis C virus. * Impossible to get enough material for at least 6 cellular vaccine production. * Absolute contraindication for the patient to receive other steps of standard treatment of glioblastoma (surgery, radio and chemotherapy)

Outcomes

Primary Outcomes

Evaluation of the treatment impact on progression-free survival

Time frame: 5 years

Secondary Outcomes

Safety evaluation

1. Direct effects attributable cell obtaining and administration 2. Adverse events during treatment 3. Neurological deterioration quantified using the NIH Stroke Scale 4. Autoimmune phenomena

Time frame: 5 years

Evaluation of impact on other efficiency clinical parameters

1. Overall survival 2. Quality of life measured with EORTC questionnaire

Time frame: 5 years

Study of specific immune response and correlates with clinical outcome

1. Delayed hypersensitivity 2. Humoral response to autologous tumor cells/tumoral lysate 3. Cellular response (proliferation, cytokine production, specific cytotoxicity)

Time frame: 5 years

Cell line characterization and correlate the final product with clinical efficacy

a. Phenotypic studies

Time frame: 5 years

Efficacy & Safety of Autologous Dendritic Cell Vaccination in Glioblastoma Multiforme After Complete Surgical Resection

Overview

Conditions

Interventions

Eligibility

Locations (1)

Outcomes

Primary Outcomes

Secondary Outcomes