Exploration of HIV Reservoirs

N/ACompletedNCT01019044

Objectif Recherche Vaccins SIDA11 enrolled

Overview

Prospective study in HIV-1 infected patients with a plasma viral load below the limit of detection and stable for at least 5 years.

Evaluation of the mucosal HIV reservoirs (HIV-DNA quantification and distribution of the infected T lymphocytes in the gut mucosa associated lymphoid tissue compared to the blood in HIV-1 infected patients under antiretroviral treatment with an undetectable plasma viral load below the 50 copies/ml limit of detection for at least 5 years).

Study Type

OBSERVATIONAL

Enrollment

Conditions

HIV-1 Infection

Interventions

Rectal mucosa biopsyPROCEDURE

Collection of a maximum of 15 rectal mucosa samples in the high portion of the rectum near the rectosigmoidal junction

Eligibility

Sex: ALLMin age: 18 Years

Medical Language ↔ Plain English

Inclusion Criteria: * HIV-1 documented infection * HIV-1 plasma viral load measurable before antiretroviral treatment initiation * Patients treated with an antiretroviral combination containing a protease inhibitor and/or a non-nucleosidic reverse transcriptase inhibitor without any interruption since treatment initiation * Patients with a stable plasma viral load below the limit of detection (HIV-RNA \< 50 copies/ml since January 2006 and/or HIV-RNA \< 200 copies/ml during the anterior period) under antiretroviral treatment for at least 5 years and for at least 90 % of the measures Exclusion Criteria: * Contraindication to the biopsy * No ability or willingness to provide informed consent * Concomitant treatment with antithrombotics or platelets antiaggregatory * Patients co-infected with HCV and or HBV * Patients who received an immunosuppressive treatment during 3 months prior enrollment (chemotherapy, radiotherapy, corticotherapy, splenectomy) or an immunotherapy during 5 years prior enrolment (IL-2, anti-HIV vaccine, IFN-alpha)

Locations (1)

Groupe Hospitalier Pitié-Salpêtrière

Paris, France

Outcomes

Primary Outcomes

Quantification of the HIV-RNA plasma viral load using ultrasensible assay (limit of detection: 1 copy/ml)