Abnormal Expression Proteins, Mitochondrial DNA and miRNA of Irritable Bowel Syndrome

Chinese PLA General Hospital180 enrolled

Overview

In the investigators study, the investigators will focus on the screening of the related proteins and miRNA to IBS in order to reveal the possible clues or molecular mechanism for this disorder.

For the proteomic procedures, Our study groups extracted proteins from the cecum and sigmoid colon samples and ran on the two-dimensional gel electrophoresis (2-DE). The proteins on 2-DE were visualized by the silver stain, and then gel maps were scanned and analyzed with the Image Master 2D Elite. The obvious abnormal protein spots were identified by the mass spectrometry. Western blot 、 immunohistochemistry 、 mRNA and miRNA were also performed for further verification of the proteins.

Study Type

OBSERVATIONAL

Enrollment

180

Conditions

Irritable Bowel Syndrome Digestive System Diseases Gastrointestinal Diseases Intestinal Diseases Colonic Diseases Colonic Diseases, Functional

Eligibility

Sex: ALLMin age: 18 YearsMax age: 60 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Patients with symptoms meeting Rome III criteria for diagnosis of IBS-C and IBS-D Exclusion Criteria: * Patients with organic illnesses or a history of major abdominal surgery * Major psychotic episode, mental retardation or dementia * Pregnant women and lactational women * Postinfectious IBS, PI-IBS

Data from ClinicalTrials.gov

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