RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This research study is looking at biomarkers in cell samples from patients with acute myeloid leukemia.
OBJECTIVES: * To determine the significance of regulation of microRNA-34a and microRNA-194 by C/EBPα during granulopoiesis in patients with acute myeloid leukemia with CEBPA mutations. * To determine whether overexpression of micro RNA-34a and microRNA-194 in myeloid progenitors with C/EBPα mutations leads to granulocytic differentiation. * To examine how microRNA-34a and microRNA-194 downregulate E2F3 during granulopoiesis. * To determine the role of microRNA-34a and microRNA-194 in myeloid cell proliferation. OUTLINE: Cryopreserved cell samples are collected for laboratory analysis, including mutation analysis, gene expression analysis, RNA analysis, and fluorescence-activated cell sorting (FACS) analysis.
Study Type
OBSERVATIONAL
Enrollment
6
Significance of regulation of microRNA-34a and microRNA-194 by C/EBPα during granulopoiesis
Relationship between overexpression of microRNA-34a and microRNA-194 in myeloid progenitors with C/EBPα mutations and granulocytic differentiation
Downregulation of E2F3 by microRNA-34a and microRNA-194 during granulopoiesis
Role of microRNA-34a and microRNA-194 in myeloid cell proliferation
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