The nature of dietary lipids, age and perivisceral adiposity could alter the postprandial inflammatory response. It is supposed that there is a relationship between the postprandial inflammatory response and the repartition of fat and lean mass, which could partially explain the muscle mass loss with age. The aim of this study is to compare the inflammatory response of a normal meal or high fat meal (from different fatty acids sources) between young lean and young obese and aged lean and aged obese men.
32 healthy volunteers will be separated into four groups: 8 young lean men, 8 young obese men, 8 aged lean men and 8 aged obese men. They will come to the center four times after inclusion, with 2 to 4 weeks wash out each time. At the first visit they will undergo different test to evaluate their body composition, glucose tolerance, resting energy expenditure, and muscular strength. At the other three visits, people will undergo a kinetic blood sampling, urine and biopsies of adipose tissue over a period of 8h after ingestion of a test meal. The different meals tested (Oleic acid vs palmitic acid vs no lipid) will be given in a random way over the three visits. Before each of these three visits, a controlled diet will be established. At the last visit body composition and muscular strength will be evaluated.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
HEALTH_SERVICES_RESEARCH
Masking
SINGLE
Enrollment
32
Mashed potatoes: Potatoes + Skim milk powder
Mashed potatoes with 1g/Kg of weight, of lipids with 75% of Oleic Acid
Mashed potatoes with 1g/Kg of weight, of lipids with 32% of palmitic acid
Human nutrition laboratory
Clermont-Ferrand, Auvergne, France
Inflammatory profile by interleukin-6 (IL6) concentration in plasma.
Time frame: six times per day (kinetic: T0 to T480) on three interventional days
Muscular strength, Body composition
Time frame: Once at the first and at the last visit
Total plasma lipids (total cholesterol, HDL-Cholesterol, LDL-cholesterol, Triglycerides (TG), non-esterified fatty acid, Plasma hormonal profile, Plasma inflammatory profile, Markers of Endothelial function, Plasma lipopolysaccharide concentration.
Time frame: Six times per day (kinetic: T0 to T480), on three interventional days
Pro-inflammatory cytokines expression in subcutaneous adipose tissue
Time frame: Twice per day (before the meal and 6 hours after), on three interventional days
Urinary metabolomic profile
Time frame: Three times per days (T0,T240,T480), on three interventional days
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