A Study to Characterize the Effect on the Pharmacokinetics of ASP1941 and Pioglitazone Hydrochloride When Given Together to Healthy Subjects

Inclusion Criteria: * The subject weighs at least 50 kg, and has a body mass index (BMI) of 18 to 32 kg/m2 inclusive * The subject's 12-lead electrocardiogram (ECG) results are normal * The female subject must be at least two years postmenopausal, surgically sterile or practicing effective birth control and not pregnant or lactating * The male or female subject agrees to practice highly effective birth control from Screening until 7 days post last dose Exclusion Criteria: * The subject has a history or evidence of any clinically significant (as determined by the investigator) cardiovascular, endocrinologic, hematologic, hepatic, immunologic, metabolic, urologic, pulmonary, neurologic, dermatologic, psychiatric, renal, and/or other major disease or malignancy (excluding non-melanoma skin cancer) * The subject has any condition possibly affecting drug absorption (e.g., gastrectomy) * The subject has history of consuming more than 14 units of alcoholic beverages per week within 6 months prior to screening or has a history of alcoholism or drug/chemical/substance abuse within past 2 years prior to screening or the subject tests positive for alcohol or drugs of abuse * The subject has/had febrile illness or symptomatic, viral, bacterial (including upper respiratory infection), or fungal (non-cutaneous) infection within 1 week prior to clinic check in * The subject has a supine mean systolic blood pressure \< 90 or \> 160 mmHg and a mean diastolic blood pressure \< 50 or \> 90 mmHg, or pulse rate higher than 100 beats per min (bpm) * The subject has a 12-lead ECG demonstrating QTc \>470 msec (female) or \>450 msec (male) * The subject is known positive for human immunodeficiency virus (HIV) antibody * The subject has a positive test for tuberculosis (TB), hepatitis C antibody, or positive for hepatitis B antigen (HBsAg) * The subject has used prescription or non-prescription drugs within 2 weeks or 5 half-lives (whichever is longer) or complementary and alternative medicines (CAM) within 14 days prior to study drug administration (excluding oral contraceptives, hormone replacement therapy \[HRT\], and acetaminophen) * The subject has had any significant blood loss, donated one unit (450 mL) of blood or more, or received a transfusion of any blood or blood products within 60 days or donated plasma within 7 days prior to clinic admission