Apoptotic Cell (AC) Uptake by Human Alveolar Macrophages (AM)

VA Office of Research and Development17 enrolled

Overview

The purpose of this study is to compare the ability of two types of white blood cells to eat dead host cells, and how this process affects their ability to protect the body from infection. The two cell types are monocytes, a cell in the bloodstream, and alveolar macrophages, a cell in the lung that is ultimately derived from monocytes

Volunteers, who may be Veterans or non-Veterans, will be screened by a series of questionnaires, chest X-ray, spirometry, EKG, blood work, and a complete history and physician examination by a study physician. Those who qualify for the study and agree via informed consent, will undergo a fiberoptic bronchoscopy on a separate day for the initial visit. Bronchoscopy is a procedure in which a flexible video instrument is passed via the nose or mouth, through the vocal cords and into the lungs. During the procedure, portions of the lungs will be washed ("lavaged") with a salt water solution, to collect the alveolar macrophages. This procedure involves conscious sedation, so that a driver is required on the day of the bronchoscopy. The entire procedure, including placement of an intravenous line to administer medications, local anesthetics, the bronchoscopy itself, and the recovery period, takes about 2.5-3 hours. The cells collected by bronchoscopy will be studied in the research laboratory, and used entirely in that procedure.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Chronic Obstructive Pulmonary Disease

Interventions

Fiberoptic bronchoscopyPROCEDURE

which a flexible video instrument is passed via the nose or mouth, through the vocal cords and into the lungs. During the procedure, portions of the lungs will be washed ("lavaged") with a salt water solution, to collect the alveolar macrophages. This procedure involves conscious sedation, so that a driver is required on the day of the bronchoscopy. The entire procedure, including placement of an intravenous line to administer medications, local anesthetics, the bronchoscopy itself, and the recovery period, takes about 2.5-3 hours.

blood drawPROCEDURE

Blood will be collected at the enrollment visit to test the subject to exclude disorders that would make it unsafe to perform the bronchoscopy, including severe anemia, infection, or disorders of kidney or liver function.

intravenous catheter

Eligibility

Sex: ALLMin age: 18 YearsMax age: 80 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Veteran or non-Veteran * Able to give informed consent Exclusion Criteria: * Unstable heart disease * Other systemic disease and unlikely to survive at least 2 years * Mental incompetence * Prednisone \>20 mg/day * Participation in another interventional protocol within last 6 weeks * Asthma * Cystic fibrosis * Clinically significant bronchiectasis * Lung cancer or any cancer not in remission for at least 5 years * Other inflammatory or fibrotic lung disease * Use of antibiotics for a lung infection within the past 4 weeks * Autoimmune disease, i.e., Rheumatoid arthritis, systemic lupus erythematosus, autoimmune hepatitis, Crohn's disease or other

Locations (1)

VA Ann Arbor Healthcare System, Ann Arbor, MI

Ann Arbor, Michigan, United States

Outcomes

Primary Outcomes

macrophage uptake of apoptotic cells in vitro

Time frame: 1-7 days

Secondary Outcomes

spirometric measurement of lung function

Time frame: 1-30 days

Data from ClinicalTrials.gov

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