Study of Pre-clearance of Latent Tuberculosis Infection And BCG Revaccination

Inclusion Criteria: 1. Males and non-pregnant, non-lactating females aged 18 to 40 years, inclusive. 2. Be available for study follow-up. 3. Be in good general health as judged by a physician on the basis of reported medical history and physical examination including blood pressure (BP) and respiratory evaluation. 4. Have a visible Bacillus Calmette-Guérin (BCG) scar. Subjects with medical documentation of BCG vaccination after 2 years of age will be excluded. 5. Have a positive (greater than or equal to 15 mm induration at 48 to 72 hours after placement) tuberculin skin test \[TST; Mantoux method using 2 tuberculin units (TU) Purified Protein Derivative (PPD) RT-23\]. 6. Weigh at least 45 kg. 7. Negative human immunodeficiency virus (HIV) approved test. 8. Demonstrate adequate understanding of the study and its requirements for participation, as demonstrated by discussions with study staff, and be able to provide written informed consent to participate in the study. 9. Females must not be pregnant, as determined by negative pregnancy test at screening, have a negative urine pregnancy test on the day of BCG revaccination, and must be non-lactating. For women of child bearing potential, use an effective contraception (licensed hormonal treatment, monogamous relationship with vasectomized partner, surgical sterilization) for 30 days prior to immunization and for the 2-year period of study follow-up. 10. Serum aspartate aminotransferase (AST) or alanine transaminase (ALT) \< 1.1 x upper limit of normal (ULN) and serum total bilirubin \< 1.1 x ULN. 11. Serum creatinine \< 1.5 mg/dL; urinalysis dipstick negative for glucose and 1 plus protein. 12. Total white blood cell (WBC) count \> 3.5 x 10\^3/mm\^3 and \< 10.8 x 10\^3/mm\^3. 13. Hemoglobin \> 12 gm/dL (female) and \> 13.5 gm/dL (male). 14. Have negative serologic tests for hepatitis B surface antigen and hepatitis C antibody. Exclusion Criteria: 1. Known hypersensitivity to Isoniazid (INH) or to any component of Bacillus Calmette-Guérin (BCG) vaccine. 2. Exposure to a case of Tuberculosis (TB) with known INH resistance. 3. Current smear or culture-confirmed or clinically diagnosed active TB 4. Suspected active TB (recurrent fever, fatigue, night sweats, weight loss, oral ulcers, diarrhea, nausea or vomiting, bleeding) or patients who are receiving anti-tuberculosis drugs. 5. Prior TB treatment, prior treatment for latent tuberculosis infection (LTBI), a self-reported history or vaccination card or medical record documenting receipt of BCG vaccination after 2 years of age. 6. Pregnant or nursing females. 7. Chronic immunosuppressive disorder or a condition requiring chronic immunosuppressive treatment such as rheumatoid arthritis or severe asthma requiring chronic corticosteroid therapy. For corticosteroids, this will mean prednisone, or equivalent, 0.5 mg/kg/day or daily use of inhaled corticosteroids. Topical steroids are allowed. 8. Active dermatitis such as atopic dermatitis. 9. Persons with any history of scarring badly or keloid formation based on physical examination. 10. Receipt of blood products or immunoglobulin within six months of BCG revaccination. 11. History of chronic illness requiring close physician follow up, including known chronic liver disease or cirrhosis, or any medical, psychiatric, occupational, or substance abuse problems that make it unlikely the volunteer will comply with the protocol as determined by the local investigator. 12. History of acute or chronic medical conditions including, but not limited to diabetes mellitus, chronic renal failure/dialysis, silicosis, gastrectomy, jejunoileal bypass, solid organ transplantation such as renal or cardiac transplants, carcinoma of the head and neck, and disorders of the liver, kidney, lung, heart, or nervous system, or other metabolic or autoimmune/inflammatory conditions. 13. Have any systemic symptoms including fever, myalgia, fatigue, chills, night sweats, weight loss, nausea, vomiting or bleeding, diarrhea, abdominal pain, rhinorrhea, cough, wheezing, or shortness of breath within 72 hours before vaccination or signs of mucosal ulceration, lymphadenitis, gastrointestinal, or pulmonary disease by physical examination on day of vaccination. Subjects with minor self-limited illnesses such as common colds or gastroenteritis may return for re-evaluation after 3 days if their symptoms have abated for vaccination. 14. Have lymphadenopathy, hepatosplenomegaly, or other abnormalities on physical examination. 15. Have close contacts, with confirmed or suspected, human immunodeficiency virus (HIV) infection or other immunodeficiency state (including diabetes mellitus, chronic dermatitis/severe eczema, chronic renal failure, hematologic malignancy), or on chronic immunosuppressive therapy. A close contact is defined as a person who lives in the same home as the subject. 16. Live in a household where any household member has been diagnosed or treated for active TB during the previous 2 years. 17. Have received or currently be receiving chronic (more than 14 days) immunosuppressive therapy within 6 months prior to screening. This includes chronic treatment with oral prednisone 0.5 mg/kg/day (or equivalent). Inhaled and topical steroids are allowed. 18. Persons with chronic heavy ethanol intake or injection drug use. Chronic heavy ethanol intake is defined as an average continuous daily intake of more than 2 drinks, or a pattern of ethanol use, which, in the opinion of the investigator would prejudice his or her participation in the trial. Injection drug use will be defined as any non-medical injection drug use during the previous 2 years. 19. Participated in another research study that includes receiving an experimental drug within 30 days prior to study entry. 20. Receipt of live attenuated vaccines within 60 days of study entry. Note: Medically indicated inactivated vaccines are not exclusionary, but should be given at least 2 weeks away from BCG revaccination, or any immunologic sampling time point post BCG revaccination. 21. Have a history of the use of a systemic antibiotic within the 14 days prior to revaccination or planned use of a systemic antibiotic in the 3 months after revaccination.