Transplantation of Cultivated Corneal Epithelial Sheet in Patients With Ocular Surface Disease

Inclusion Criteria: 1. Male or non-pregnant females between 18 and 75 years of age. 2. Written informed consent obtained from patient or parents/guardian. 3. Patients with unilateral limbal stem deficiency 2-12 months presenting with any of the following * Conjunctivalisation * Absence of limbal palisades of Vogts * Chronic inflammation * Persistent or recurrent corneal epithelial defect 4. Patients who had 2-12 months of conservative treatment. \* Diagnostic criteria for limbal stem cell deficiency are as follows: * Symptoms of decreased vision, redness, watering, photophobia and recurrent attacks of pain * Triad of signs: conjunctivalisation, neovascularisation and chronic inflammation * Stippled appearance of cunjunctivalised cornea with loss of palisades of Vogt * Recurrent and persistent epithelial defects * Superficial vascularisation, scarring, thick fibrovascular pannus, ulceration, melting and perforation Exclusion Criteria: Patients with any of the following are not eligible for enrollment into the study: 1. Pregnant or nursing woman or women of childbearing potential except if post-menopausal, surgically sterile or using accepted method(s) of birth control or having negative pregnancy test. 2. Participation in any drug trial in which the patient received an investigational drug within 30 days or 5 half-lives (whichever is longer) preceding the screening phase of this study. 3. Those persons directly involved in the conduct of the study. 4. Any history of severe infection such as hepatitis, renal, gastrointestinal, endocrine or neurological disease. 5. Evidence of corneal stromal scarring, cataract, macular oedema or scarring, retinal detachment and conjunctival keratinisation 6. Positive for HIV, Hepatitis B, C and VDRL 7. History of Pulmonary tuberculosis, hepatitis B, 8. History of alcohol or substance abuse 9. History of malignancy within previous 5 years 10. History of organ transplant 11. Any serious medical conditions or disability, which in the opinion of the investigator, would interfere with treatment or assessment or preclude completion of this study.