The purpose is to investigate the electroretinogram (ERG) in young, healthy men in the normoglycaemic and hyperglycaemic state before and after intervention with corticosteroids treatment, high calorie diet and exercise restraint.
The subjects will be fasting from midnight the day before the experiment. Standard procedures (including pupil dilation, dark adaptation, and local anaesthetics) are carried out to allow the ERG to be obtained. After electrophysiology, the subject will be clamped at a plasma glucose level of 10 mM (\~180 mg/dL) and after a stabilisation period the ERG protocol is repeated. Each subject is examined twice on two different days. The first day the subjects are normoglycaemic in the morning (first examination) and hyperglycaemic (clamped to 10 mM) throughout the second examination. The second day subjects are also normoglycaemic and hyperglycaemic but shows up after 7-12 days of daily intake of prednisolone, high calorie diet, and exercise restraint.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
10
12 days of corticosteroids treatment (prednisolone 37,5 mg/day)
High calorie diet (130 % of recommended daily energy intake) and relative physical inactivity (no exercise and at least 8 hours of rest/day)
Copenhagen University Hospital at Glostrup
Copenhagen, Copenhagen, Denmark
ERG
Characteristics of the scotopic ERG at normo-/hyperglycaemia before vs. after prednisolone treatment
Time frame: 12 days
Retinal Vessel Caliber
Measurement of retinal vessel caliber from fundus photographs before and after treatment with glucocorticoids.
Time frame: 12 days
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.