The purpose of this study is to study impairment of white blood cell function in patients with type II diabetes.
Leucocytes from poorly controlled diabetes exhibit aberrant chemotaxis, increased susceptibility to bacterial infection, leukotriene production, lysosomal enzyme release, proinflammatory cytokine expression and production of reactive oxygen species. Aberrant glucose concentration in diabetics affects functions of peripheral blood system as well as the immune system leading to impaired host defense. Impaired wound healing is a serious complication associated with diabetes. We hypothesized that impairment in leukocyte function results in dysfunctional inflammatory response in diabetic wounds. The proposed studies focus on characterizing mechanisms that will improve our understanding of the dysfunctional inflammatory response resulting in non-healing chronic wounds in diabetics.
Study Type
OBSERVATIONAL
Enrollment
42
Ohio State University Comprehensive Wound Center
Columbus, Ohio, United States
Ex vivo leukocyte function by measuring ROS production
After blood draw monocytes are separated from whole blood and production of oxidants by these cells
Time frame: immediately after blood draw
Ex vivo NADPH oxidase gene and protein expression
Gene and protein expressions are measured using Western blot and real time PCR.
Time frame: After blood draw
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