VA-006 Vaccinia Vaccine (ACAM2000) for the Production of VIGIV

Inclusion Criteria: * Signed written informed consent. * Age 18 - 65 years. * Normal and healthy as determined by medical history, physical exam, vital signs and clinical laboratory tests at screening visit. * Subject must meet all required subject suitability criteria that pertain to normal Source Plasma donors. * Subject must have been previously immunized for smallpox, at ≥1 year prior to commencement of screening assessments for the VA-006 trial, and vaccination history must be confirmed by oral or written history AND the presence of a visible pathognomonic smallpox vaccination scar. * Female subjects of childbearing potential must use at least one of the following means of birth control documented by a physician's letter: * Surgical sterilization * Hormonal (oral/injectable/implant) for at least 30 days prior to vaccination * Intrauterine device (IUD) inserted at least 7 days prior to vaccination. * Female subjects who are not using one of the methods of birth control listed above must be documented as postmenopausal, which is defined as not having a menstrual period for longer than 12 months and having a serum follicle-stimulating hormone (FSH) level ≥ 40 mIU/mL. Exclusion Criteria: * History of severe adverse event(s) from previous participation in the VA-001 or VA-005 trials or any other smallpox vaccination program/study. * Subject, household contact, or other close/intimate contact: * with eczema, history of eczema, exfoliative skin conditions, wounds, burns, or other skin conditions at the Investigator's discretion. * with a history of immunodeficiencies (see section 7.1.2 of the protocol). * who received radiotherapy or chemotherapy, adrenocorticotropic hormone (ACTH), corticosteroids, or immunosuppressive drugs. * with eye disease treated with topical steroids. * with known or suspected disorders of immunoglobulin synthesis. * with leukemia, lymphomas of any type, melanoma, or other malignant neoplasms affecting the bone marrow or lymphatic systems. * with a history of adverse reactions to smallpox (vaccinia) vaccine. * has recently been diagnosed with cancer and who will be undergoing chemotherapy or radiation therapy during the vaccination healing time. * is a transplant recipient (except for corneal transplant). * is pregnant, planning pregnancy or breast feeding (female subjects must have negative serum pregnancy test at screening and negative urine pregnancy test prior to vaccination). * Household or other close/intimate contact(s) under the age of 12 months. * History of allergies to latex, phenol, any of the antibiotics listed in the vaccine content, or any other component of ACAM2000 or its diluent * Severely or morbidly obese or higher obesity classification (BMI ≥ 35). * Subjects with abnormal EKG and/or cardiac Troponin levels at screening. * Subjects with cancer or kidney disease (except kidney stones). * Subject has 3 or more of the following risk factors: * High blood pressure diagnosed by a doctor * High blood cholesterol diagnosed by a doctor * Diabetes or high blood sugar diagnosed by a doctor * A first degree relative (for example, mother, father, brother, sister) who had a heart condition before the age of 50 * Currently smokes cigarettes * Cardiovascular disease or heart condition diagnosed by a doctor at any time in the past, with or without symptoms, including: * Arrhythmia * Syncope * Previous myocardial infarction * Angina * Coronary artery disease * Congestive heart failure * Cardiomyopathy * Stroke or transient ischemic attack * Chest pain or shortness of breath with activity (such as walking up stairs) * Other heart conditions being treated by a physician.