The primary purpose of this study is to compare the change in blood carotene concentration between a food-based versus nutritional supplement-based intervention. This study will randomize up to 60 healthy volunteers to receive either carotenoid-enriched food; natural, mixed carotenoid supplementation; chlorophyll complex or placebo supplementation. Blood carotene levels will be measured before and after 28 days of supplementation. Preliminary data will also be collected on several biomarkers associated with vascular inflammation and endothelial dysfunction. These biomarkers will be measured before and during a fast food control meal at the beginning and end of the intervention period.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
TRIPLE
Enrollment
60
One capsule, twice daily with meals for 28 days
One capsule per day with meals for 28 days
Single carotenoid-enriched soup or salad serving eaten daily
Safflower oil-filled capsules, one twice daily with meals
Bastyr University Clinical Research Center
Kenmore, Washington, United States
University of Washington Clinical Research Center
Seattle, Washington, United States
Change in serum carotenoid fraction concentrations between study arms
Time frame: Following 28 day intervention
Change in oxidized LDL lipoproteins (oxLDL)
Time frame: Following 28 day intervention
Change in serum gamma-glutamyl transferase (GGT)
Time frame: Following 28 day intervention
Change in urinary isoprostanes
Time frame: Following 28 day intervention
Change in serum antioxidant capacity (ORAC)
Time frame: Following 28 day intervention
Change in lipid profile (LDL, HDL, triglycerides)
Time frame: Following 28 day intervention
Change in C-reactive protein (CRP)
Time frame: Following 28 day intervention
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