Intact Vagal Innervation for and Glucagon-like Peptide-1 (GLP-1) Effects

University Hospital, Gentofte, Copenhagen30 enrolled

Overview

The aim of this study is to investigate the role of transmission via the vagal nerve for the effect of glucose and Glucagon-like peptide-1 (GLP-1) in respect to insulin secretion. The hypothesis is that a great deal of the effects of GLP-1 is mediated via the nervous system and for this reason the investigators will research individuals with and without intact nervous supply.

GLP-1 is a potent enterogastron and incretin hormone. It is rapidly inactivated by dipeptidyl peptidase IV so only 10-15% enters the systemic circulation. This has led to the hypothesis that GLP-1 interact locally with afferent sensory nerve fibers. We investigated the role of intact vagal innervations on the effect of glucose and GLP-1 on the insulin secretion

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Vagotomy, Truncal

Interventions

Dipeptidyl peptidase 4 (DPP 4) inhibitorDRUG

One tablet (50 mg)of DPP4 inhibitor is to be taken 12 and 1 hours before start of the oral glucose tolerance test (50 g glucose and 1.5 g paracetamol dissolved in 300 ml water)on day 3

oral glucoseOTHER

50 g glucose dissolved in 300 ml water with 1,5 g paracetamol is to be ingested orally within the first 15 minutes.

Eligibility

Sex: ALLMin age: 18 YearsMax age: 90 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * normal fasting plasma glucose * normal hemoglobin * informed consent Exclusion Criteria: * type 1 diabetes mellitus or type 2 diabetes mellitus * body mass index \> 30 * inflammatory bowel disease * intestinal surgery * serum creatinine \> 250 µM and/or albuminuria * ALAT \> 2 x normal value * Severe cardiac insufficiency * in treatment with medicine which cannot be paused for 12 hours

Locations (1)

Department of internal medicine F´laboratory, Gentofte Hospital

Hellerup, Copenhagen, Denmark

Outcomes

Primary Outcomes

insulin secretion

The insulin secretion during a four-hour oral glucose tolerance test (OGTT) and an intravenous isoglycaemic clamp is evaluated

Time frame: four hours

Secondary Outcomes

plasma GLP-1

12 blood samples will be drawn during the four hours OGTT and intravenous isoglycaemic clamp, most frequently during the first hour