The purpose of this study is to learn more about why some obese persons are resistant to developing obesity-related metabolic diseases (such as diabetes and cardiovascular disease), while others are prone to developing these conditions. We will do this by studying obese persons before and after a 5% body weight gain. Subjects will be asked to increase their current diet for a period of 8-12 weeks in order to increase their current body weight by 5%. Each will then be asked to maintain this weight increase for 3 weeks. We will monitor subjects throughout this time period with weekly medical evaluations. At the completion of the study, we will provide each subject with a 6-month weight loss program.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
71
Subjects will begin an 8-12 week high-calorie diet intervention. They will eat an additional 1000 kcal/day for two to three months, until a moderate, approximately 5% weight gain is achieved. The recommended dietary energy intake will be 1000 kcal/d more than the subject's baseline resting energy expenditure. An individualized diet plan will be developed for each subject by the study dietitian based on estimated energy requirements, and the subject's food preferences and dietary habits.
Washington University School of Medicine
St Louis, Missouri, United States
Change in Intrahepatic Triglyceride
Time frame: an average of three months, from baseline to 5% weight gain
Change in de novo lipogenesis
Time frame: an average of three months, from baseline to 5% weight gain
Change in VLDL kinetics
Time frame: an average of three months, from baseline to 5% weight gain
Change in hepatic insulin sensitivity
Time frame: an average of three months, from baseline to 5% weight gain
Change in skeletal muscle insulin sensitivity
Time frame: an average of three months, from baseline to 5% weight gain
Change in adipose tissue insulin sensitivity
Time frame: an average of three months, from baseline to 5% weight gain
Change in CD36 concentration in skeletal muscle
Time frame: an average of three months, from baseline to 5% weight gain
change in CD36 concentration in adipose tissue
Time frame: an average of three months, from baseline to 5% weight gain
Change in cell proliferation (growth) rates in the colon
Time frame: an average of three months, from baseline to 5% weight gain
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