Biomarkers in Tissue Samples From Young Patients With Acute Myeloid Leukemia

Children's Oncology Group32 enrolled

Overview

RATIONALE: Studying samples of tissue from patients with cancer the in laboratory may help doctors identify and learn more about biomarkers related to cancer. PURPOSE: This research study is studying biomarkers in tissue samples from young patients with acute myeloid leukemia.

OBJECTIVES: * To determine whether the pattern of global and TSG-specific promoter CpG island hypermethylation and gene silencing that we have shown characterizes MLL-rearranged (MLL-r) infant bilineage ALL is also characteristic of other subsets of MLL-r leukemia. OUTLINE: Previously collected cryopreserved cells from diagnosis are analyzed for promoter methylation via HELP arrays, gene expression arrays, and RT-qPCR. PROJECTED ACCRUAL: A total of 32 samples (8 from each of 4 biologically defined cohorts) will be analyzed.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Leukemia

Interventions

DNA methylation analysisGENETIC

gene expression analysisGENETIC

microarray analysisGENETIC

reverse transcriptase-polymerase chain reaction

Eligibility

Sex: ALLMin age: 1 YearMax age: 18 Years

Medical Language ↔ Plain English

DISEASE CHARACTERISTICS: * Available cryopreserved cells from diagnosis * At least 2 x 10\^7 viably cryopreserved cells * One of the following biologically defined cytogenetics/molecular cohorts: * t(9;11) * t(11;19) * Other 11q23 translocations * Normal cytogenetics PATIENT CHARACTERISTICS: * Not specified PRIOR CONCURRENT THERAPY: * Not specified

Outcomes

Primary Outcomes

Identification of differential patterns of promoter hypermethylation and gene expression in pairwise comparisons with other cohorts and normal controls

Data from ClinicalTrials.gov

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