The purpose of this study is to establish if cells present in lung tissue during extrinsic allergic alveolitis (EAA) are able to elicit an immune response against specific antigens. The hypothesis is: cells in BAL lavage fluid from patients with EAA will be responsive to specific antigens and this response can be measured using ELISPOT.
Subjects suffering from extrinsic allergic alveolitis caused by pigeons will undergo a broncho alveolar lavage. Cells obtained from this BAL will be used for ELISPOT technique with pigeon specific antigens
Study Type
OBSERVATIONAL
Enrollment
10
Diakonessenhuis Utrecht
Utrecht, Netherlands
ELISPOT response in cells obtained from broncho-alveolar lavage
All elispots will be performed the same day, the end point will be available the next day
Time frame: 2 days
Elispot response in cells from blood
ELispots will be performed the same day as blood collection, results will be available the next day
Time frame: 2 days
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