Chronic Presence of Epstein Barr Virus in Epithelial Cells From Gingiva is Associated With Periodontitis

Centre Hospitalier Universitaire de Nice23 enrolled

Overview

The etiopathogenesis of periodontal disease results from complex interaction between infectious agents, mainly including bacteria, and host cellular and humoral immune responses. However it is thought that bacteria-induced pathogenesis is not sufficient alone to explain all biological and clinical features of the destructive periodontal disease. The main hypothesis is that herpesviruses, such as Epstein-Barr Virus, may participate as well by altering epithelial gingival cell biology and consequently may promote the initiation and progression of periodontitis.

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

HEALTH_SERVICES_RESEARCH

Masking

NONE

Enrollment

Conditions

Chronic Periodontitis Aggressive Periodontitis Presence / Absence of EBV

Interventions

The objective of root planing, otherwise known as conventional periodontal therapy, non-surgical periodontal therapy, or dental prophylaxis is to remove or eliminate the etiologic agents which cause inflammation: dental plaque, its products and calculus,\[1\] thus helping to establish a periodontium that is free of disease.\[2\]

dental root planingPROCEDURE

The objective of root planing, otherwise known as conventional periodontal therapy, non-surgical periodontal therapy, or dental prophylaxis is to remove or eliminate the etiologic agents which cause inflammation: dental plaque, its products and calculus, thus helping to establish a periodontium that is free of disease.

dental root planingPROCEDURE

The objective of root planing, otherwise known as conventional periodontal therapy, non-surgical periodontal therapy, or dental prophylaxis is to remove or eliminate the etiologic agents which cause inflammation: dental plaque, its products and calculus, thus helping to establish a periodontium that is free of disease.

Eligibility

Sex: ALLMin age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * 10 patients affected by chronic periodontitis * 10 patients affected by aggressive periodontitis * 10 healthy patients (orthodontics extraction) * Subject who have read and understood the information note relative under investigation and who have signed the informed consent * major patient Exclusion Criteria: * Patient with no contre-indication to periodontal treatment * Patient with serious blood disease * Patient who exhibits systemic condition incompatible with realization of the study * Patient treated with oral retinoids, bisphosphonates, oral anticoagulants and anticonvulsivants * Patient who presents a buccal carcinoma treated with radiotherapy * Patient who have received during the past 6 months: anti-inflammatory drugs, anti-cancerous or immunosuppressive chemiotherapy * Patient who presents a linguistic or psychic incapacity to understand information

Outcomes

Primary Outcomes

Percentage of gingival epithelial cells infected by EBV (by immunofluorescence and In situ Hybridization)

Time frame: at time=0

Secondary Outcomes

Epithelial tissue infected by EBV (by immunofluorescence and in situ hybridization)

Time frame: at time = 0

Presence / absence of latency and / or lytic proteins (by immunofluorescence and in situ hybridization)