Previous studies have shown that consumption of a low calorie cranberry beverage imparts a favorable impact on HDL cholesterol, LDL oxidation, and cell adhesion molecules in men. However, these studies were not well controlled. Thus, it is important to confirm these effects in a blinded, placebo-controlled feeding study. The investigators goal is to verify the cardioprotective effects of cranberries by conducting a dietary intervention trial with healthy subjects in a controlled environment.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Masking
DOUBLE
Enrollment
60
base diet supplemented with two 8 ounce servings of low calorie cranberry juice per day
base diet supplemented with two 8 ounce servings of a color and flavor matched placebo beverage
USDA's Beltsville Human Nutrition Research Center
Beltsville, Maryland, United States
Change in biomarkers of cardiovascular disease and polyphenol absorption
A blood lipid panel will include plasma total cholesterol, HDL cholesterol, LDL cholesterol, triglycerides, Apo A-1, Apo A-II, Apo B, Lpa. Inflammatory markers (IL-6, IL-10, IL-1-alpha, CRP, TNF-alpha, fibrinogen, iCAM) will be determined by ELISA. Serum will be analyzed for polyphenol concentration.
Time frame: Days 1&2; Days 27&28; Days 55&56
Change from baseline in systolic and diastolic blood pressure
Blood pressure will be measured using a standardized protocol at the beginning, 4-week, and 8-week time points.
Time frame: Days 1, 28, and 56
Change in urine metabolomics and adhesion analysis
Urine (24 hour cumulative and spot)will be collected at the beginning and end of each treatment period and provided to Ocean Spray for metabolomics and adhesion analysis.
Time frame: Days 1 & 56
Change in fecal microbiota
A spot fecal sample will be collected at the beginning and end of each treatment period and provided to Ocean Spray microbiota analysis.
Time frame: Days 1 & 56
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