TAK-329 Glucose Clamp Study

Inclusion Criteria: 1. In the opinion of the investigator, the participant or legal guardian is capable of understanding and complying with protocol requirements. 2. The participant or, when applicable, the participant's legally acceptable representative signs and dates a written, informed consent form and any required privacy authorization prior to the initiation of any study procedures. 3. Is an adult female or an adult male with Type 1 Diabetes Mellitus (diagnosed at least 1 year prior to Screening) with a glycosylated hemoglobin ≤10.0%. 4. Is aged 18 to 50 years, inclusive, at the time of informed consent and first dose of study drug. 5. Weighs at least 50 kg and has a body mass index between 18.0 and 32.0 kg/m2, inclusive at Screening. 6. Males who are nonsterilized and sexually active with a female partner of childbearing potential agree to use adequate contraception from signing of informed consent throughout the duration of the study and for 12 weeks after the last dose. 7. A female participant, including women of childbearing potential, who is sexually active with a nonsterilized male partner agree to use consistently use contraception from signing of informed consent throughout the duration of the study. 8. Was treated with insulin either in the form of multiple daily injections or as continuous subcutaneous insulin infusion (CSII or "insulin pump") for at least 3 months prior to Screening. 9. Has a fasting serum C-peptide ≤0.2 nmol/L at Screening. 10. The participant, if taking concomitant medications for stable disease, has been on a stable dose for a minimum of 60 days prior to Day 1 of Period 1 and the medications are approved by the investigator and the Takeda Medical Monitor. 11. A female participant of child-bearing potential, if using hormonal contraception, has been on stable hormonal contraception (without changes to the dose or type of preparation) for at least 6 months prior to Screening. Exclusion Criteria: 1. The participant is participating in another investigational study or has taken any investigational drug within 30 days prior to Day -1. 2. Has donated blood or experienced acute blood loss (including plasmapheresis) of more than 500 mL within 56 days prior to Day -1. 3. Has a history or clinical manifestations of clinically significant metabolic (including Type 2 Diabetes Mellitus, hypercholesterolemia or dyslipidemia but excluding Type 1 Diabetes Mellitus), endocrinologic, hematologic, pulmonary, cardiovascular, gastrointestinal, neurologic, hepatic, renal, urologic, immunologic, glaucoma, or psychiatric disorders, or tendency for urinary retention. 4. Has a known hypersensitivity to TAK-329, any of its excipients, any compound related to TAK-329, or known hypersensitivity to sulfonamides. 5. Has a history of cataract (including traumatic) or glaucoma or has a positive ophthalmic examination with findings suggestive of a cataract within 7 days prior to or at Check in (Day -1) of Period 1. 6. Has a history indicative of proliferative retinopathy or maculopathy and/or severe neuropathy including gastroparesis. 7. Has a history of severe hypoglycemia requiring emergency medical attention less than 6 months prior to Screening or a medical history suggestive of hypoglycemia unawareness less than 1 year prior to Screening. 8. Has a systolic blood pressure ≥145 mm Hg or a diastolic blood pressure ≥90 mm Hg that is confirmed by a repeat measurement taken at least 30 minutes apart at Screening and Check-in (Day -1) of Period 1. 9. Has a history of drug abuse (defined as illicit drug use) or a history of alcohol abuse (defined as regular or daily consumption of more than 4 alcoholic drinks per day) within 2 years prior to Screening. 10. Has an alanine transaminase or aspartate transaminase level of greater than 1.5 x the upper limit of normal at Screening or Check-in (Day -1), active liver disease, active gall bladder disease, or jaundice 11. Has a thyroid stimulating hormone result out of the euthyroid range at Screening. 12. Is on any non-insulin antidiabetic medication (including oral agents such as metformin or pioglitazone or injectables such as pramlintide or exenatide) or inhaled insulin within 90 days prior to Day -1 of Period 1. 13. Has ingested foods or beverages containing grapefruit juice or Seville-type oranges within 14 days prior to Day 1 of Period 1 or any alcohol-or caffeine containing products or medications within 72 hours prior to Day 1 of each treatment period or anticipates an inability to abstain from these substances for the duration of the study. 14. Has used any drug within 28 days prior to Day 1 of Period 1 that may interfere with the interpretation of study results or are known to cause clinically relevant interference with insulin action or glucose utilization. 15. Has a history of cancer that has not been in remission for at least 5 years prior to the first dose of study drug. (This criterion does not include those subjects with basal cell or stage I squamous cell carcinoma of the skin.) 16. Has a history of organ transplant including islet cells transplantation. 17. Has a known history of human immunodeficiency virus or a positive hepatitis B surface antigen and antibody to hepatitis C result at Screening. 18. Has a positive urine screen for drugs or alcohol breathalyzer at Screening or Day -1 of Period 1. 19. Has any other disease or condition including psychiatric conditions at Screening or at Randomization that, in the opinion of the investigator, may adversely affect his or her participation in the study either by being a safety risk to the subject, affecting any of the pharmacokinetic or pharmacodynamic measurements, or making it difficult to successfully manage and follow the subject according to the protocol. 20. Has a history of abdominal surgery (except laparoscopic cholecystectomy or uncomplicated appendectomy), thoracic, or nonperipheral vascular surgery within 6 months prior to Day -1 of Period 1. 21. Has a history or presence of an abnormal electrocardiogram result at Screening or Day -1 of Period 1 that, in the investigator's opinion, is clinically significant. 22. Has used any tobacco within 28 days prior to Day 1 of Period 1 OR has a positive urine cotinine test at Screening, and is unwilling to abstain from these products for the duration of the study. 23. Has poor peripheral venous access. 24. Has had an acute, clinically significant illness within 30 days prior to Day -1, or any other condition or prior therapy that, in the opinion of the investigator, would make the subject unsuitable for the study. 25. The participant is a study site employee, or an immediate family member (ie, spouse, parent, child, or sibling) of a study site employee involved in the conduct of this study. 26. The participant had an acute, clinically significant illness within 30 days prior to Day -1, or any other condition or prior therapy that, in the opinion of the investigator, would make the subject unsuitable for the study. 27. The participant is unable to understand verbal or written English or any other language for which a certified translation of the approved informed consent is available.