Study to Identify Biomarkers for Protein Intake

Wageningen University25 enrolled

Overview

Results from observational studies suggest a small beneficial association between protein and blood pressure that may be mainly attributable to the intake of protein from plant sources. In epidemiological studies biomarkers of dietary intake are useful to estimate intake more reliably. Dietary studies using biomarkers of protein intake generally show stronger associations with health outcomes. However, data on biomarkers for specific types of protein (e.g. from dairy, meat, grain and legumes) are scarce. Therefore the purpose of this study is to identify new biomarkers for the intake of protein from meat, dairy and grain. In addition the secondary purpose is to investigate whether intake of protein from these sources influences kidney filtration rate and urinary acid excretion differentially.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Masking

NONE

Enrollment

Conditions

Identify New Biomarkers in Blood or Urine After Consumption of Meat Protein, Dairy Protein and Grain Protein in Healthy Subjects.

Interventions

Eligibility

Sex: ALLMin age: 18 YearsMax age: 40 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Age 18-40 years * BMI between 18.5 and 30 kg/m2 * Healthy (As judged by the participant) * Written informed consent obtained Exclusion Criteria: * Unable or unwilling to comply with study procedures. * Unusual dietary patterns, including high alcohol intakes (≥ 2 glasses/day) and a vegetarian diet * Recent (\< 4 weeks) or current participation in a study with any investigational drug or dietary intervention * Usage of an energy restricted diet during the last two months * Weight loss or weight gain of 5 kg or more during the last two months * Smoking * Stomach or bowel diseases * Kidney disorders * Diabetes, thyroid disease, other endocrine disorders * Prevalent cardiovascular disease * Extreme sports (\>8h/ wk) * Usage of any prescribed medication except for oral contraceptives * Use of systemic antibiotics in the period of 3 month prior to the study * For women: pregnant or lactating * For women: not using oral contraceptives * For women: Unwilling to use oral contraceptives for consecutively for at least the total study duration * Liver function parameters (ALAT, ASAT, and γ-GT) and renal function parameters (Serum creatinin) outside the normal range

Outcomes

Primary Outcomes

Differences between dietary periods in concentrations of potential biomerkers in blood and urine

Potential biomarkers that will be measured are urinary creatinin, sulphate, carnosine, 1-MH, 3-MH, taurine,14N/15N and creatin in blood. Furthermore, amino acid profiling will be done in blood and urine to detect differences between dietary periods

Time frame: On the last day of every one week intervention period

Secondary Outcomes

Differences between dietary period in estimated renal filtration

Renal filtration will be estimated using bromide distribution in combination with bio-impedance measurement

Time frame: blood and urine collection on the last day of each intervention period

Differences between dietary periods in urinary acid excretion

Time frame: urine collection on the last day of each intervention period