The objective of this study is to investigate the potential beneficial effect of increasing protein in the diet in order to decrease hepatic lipid accumulation on a high-fat diet. The investigators hypothesize that increasing protein in a high-fat diet suppresses lipid accumulation in the liver, and that changes in (hepatic) fat handling underlie this reduced lipid accumulation.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
SINGLE
Enrollment
29
in the low-protein group 13EN% of protein will be provided in the diet; in the high-protein 25EN% of protein will be provided
The control group will get a diet which is according to healthy eating guidelines.
Wageningen University, Division of Human Nutrition
Wageningen, Gelderland, Netherlands
hepatic fat accumulation
Time frame: baseline, 2 weeks, 4 weeks
Biomarkers of liver function/hepatic steatosis
Biomarkers of liver function/hepatic steatosis: ALT, AST, C-reactive protein
Time frame: baseline, 2 weeks, 4 weeks
Circulating cytokines
adiponectin, TNF-α
Time frame: baseline, 2 weeks, 4 weeks
Postprandial lipid metabolism
Postprandial lipid metabolism will be assessed by means of a meal challenge with the use of stable isotope tracer
Time frame: 2 weeks, 4 weeks
Glucose homeostasis
Glucose homeostasis will be assessed with the homeostatic model assessment (HOMA) index in fasting blood samples. In addition dynamic indexes will be determined from the meal challenge.
Time frame: baseline, 2 weeks, 4 weeks
Adipose tissue gene expression
Time frame: 2 weeks, 4 weeks
Peripheral blood mononuclear cells gene expression (PBMC's).
Time frame: baseline, 2 weeks, 4 weeks
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