The aim of this study is evaluated the effect of dietetic supplementation with DHA on human sperm quality, antioxidant capacity and sperm DNA damage.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
DOUBLE
Enrollment
120
1500 mg sunflower/day for 10 weeks
1500mg/day for 10 weeks
Instituto Valenciano de infertilidad
Murcia, Murcia, Spain
sperm concentration
million of spermatozoa per milimitre
Time frame: 5 weeks
Sperm motility
Percentage of motile sperm.
Time frame: 5 weeks
Sperm morphology
Percentage of sperm with normal morphology
Time frame: 5 weeks
Total capacity antioxidant
Total antioxidant capacity (TAC)is a colorimetric assay that measure the combined antioxidant activities of all its non enzymatic constituents in seminal plasma.
Time frame: 5 weeks
Sperm DNA damage.
DNA fragmentation analysis was evaluated by TUNEL test and measured by flow cytometry
Time frame: 5 weeks
Fatty acids measure.
Fatty acid analysis in seminal plasma and spermatozoa were evaluated by gas chromatography.The percentage of each fatty acid class was expressed as percentage of total fatty acids.
Time frame: 5 weeks
Sperm in ejaculate
Million of spermatozoa in eyaculate.
Time frame: 5 weeks
Volume of ejaculate.
Eyaculated milliliters.
Time frame: 5 weeks
Sperm viability
Porcentage of sperm whitout membrane damage
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Time frame: 5 weeks