Effect of Platelet Rich Plasma on Photoaged Skin

Inclusion Criteria: Part 1: * Subjects of either gender (M/F), 35-60 years old. * Subjects are in good health. * Subjects who are willing and have the ability to understand and provide informed consent for participation in the study and are able to communicate with the investigator. Part 2: * Subjects of either gender (M/F), 35-60 years old. * Subjects are in good health. * Bilateral cheek wrinkles "in motion" with severities of ≥ type II of Glogau photoaging classification (APPENDIX I). * Subjects who are willing and have the ability to understand and provide informed consent for participation in the study and are able to communicate with the investigator. * Subject requests cosmetic improvement of facial wrinkles. Exclusion Criteria: * Pregnant or lactating. * Subjects who are unable to understand the protocol or to give informed consent. * Subjects who have a self-reported history of blood or platelet disorders e.g. anemia, thrombocytopenia, coagulopathy, hypofibrinogenemia, or are on anticoagulant or antiplatelet therapy. * Subjects who have active skin disease or skin infection in treatment area. Subjects who have a history of hypertrophic scars and keloids. * Subjects who have any requirement for the use of local or systemic steroids or immunosuppressive agents. * Subject notes that he/she is HIV positive * Subjects with history of skin cancer or actinic keratosis * Uncooperative patients or patients with neurological disorders who are incapable of following directions or who are predictably unwilling to return for follow-up examinations. * Skin conditions that interfere with wrinkle assessment/treatment (excessive dermatochalasis, inability to lessen the wrinkles by physically spreading the area apart). * Excessive exposure to the sun, such as jobs requiring constant outdoor exposure. * Known genetic disorders affecting fibroblasts or collagen, such as achondroplasia, osteogenesis imperfecta, ect. Part 2: * Pregnant or lactating. * Subjects who are unable to understand the protocol or to give informed consent. * Subjects who have a history of blood or platelet disorders e.g. anemia, thrombocytopenia, coagulopathy, hypofibrinogenemia, or are on anticoagulant or anti-platelet therapy. * Subjects who have had topical or oral tretinoin, chemical peeling, botulinum toxin injection or laser and light treatment for facial rhytides or rejuvenation within past 6 months or planning to undergo treatments as described in the next 3 months. * Facial surgery in the lower 2/3 of the face or semi-permanent dermal fillers within 1 year prior to study enrollment * Subjects who have history of recurrent facial or labial herpes simplex infection. * Subjects who have active skin disease or skin infection in treatment area. * Subjects who are allergic to lidocaine or prilocaine. * Subjects who have a history of hypertrophic scars and keloids. * Subjects who have any requirement for the use of local or systemic steroids or immunosuppressive agents. * Subject notes that he/she is HIV positive * Subjects with history of skin cancer or actinic keratosis * Uncooperative patients or patients with neurological disorders who are incapable of following directions or who are predictably unwilling to return for follow-up examinations. * Skin conditions that interfere with wrinkle assessment/treatment (excessive dermatochalasis, inability to lessen the wrinkles by physically spreading the area apart). * Excessive exposure to the sun, such as jobs requiring constant outdoor exposure. * Known genetic disorders affecting fibroblasts or collagen, such as achondroplasia, osteogenesis imperfecta, ect.