Understanding Typhoid Disease After Vaccination

Inclusion criteria: * Male or female aged 18 - 60 years inclusive and in good health. * Have an abdominal ultrasound scan result documented demonstrating no evidence of gallbladder pathology. * Willing to allow their general practitioner and/or hospital consultant (if relevant) and the Health Protection Unit to be notified of participation in the study. * Agree to refrain from blood donation in the future if diagnosed with typhoid fever. * Be willing to have 24-hour contact with study staff during the four weeks post-challenge. Exclusion Criteria: * Have previously received any typhoid vaccine, been resident in a typhoid endemic country for over 6 months, been diagnosed with probable or confirmed typhoid infection or been challenged with Salmonella Typhi or enrolled in a typhoid challenge study. * Have any known or suspected impairment or alteration of immune function. * History of significant cardiovascular disease. * History of significant respiratory disease. * History of significant endocrine disorder. * History of significant renal or bladder disease. * History of biliary tract disease. * History of significant gastrointestinal disease. * History of significant neurological disease. * History of significant metabolic disease. * History of significant haematological diagnosis. * History of psychiatric illness requiring hospitalisation, current known or suspected drug or alcohol misuse. * History of significant infectious disease. * History of non-benign cancer. * Presence of any implants or prostheses. * Hypersensitivity to any component of the vaccine or are hypersensitive to two or more of the following antibiotics: ciprofloxacin, azithromycin, ampicillin, trimethoprim sulfamethoxazole. * Female participant who is pregnant, lactating or who is unwilling to ensure that they or their partner use effective contraception one month prior to vaccination and continue to do so until two negative stool samples obtained a week apart, a minimum of 1 week after completion of antibiotic treatment have been obtained. * Current occupation involving: clinical or social work with direct contact with young children (defined as those attending pre-school groups, nursery or aged less than 2 years); highly susceptible patients or persons in whom typhoid infection would have particularly serious consequences (i.e. those who are immunocompromised or debilitated); care work involving the elderly. * Current occupation as a commercial food handler involving the preparation or serving of unwrapped foods not subjected to further heating. * Household contact with a young child (defined as above). * Household/close contact who is immunocompromised. * Scheduled elective surgery or other procedures requiring general anaesthesia during the vaccine/challenge period, at time of enrolment. * Participants who have taken part in other research involving an investigational product (IMP) within the 30 days prior to enrolment. * Have received blood, blood products and/or plasma derivatives including parenteral immunoglobulin preparations in the previous 3 months * Any other significant disease or disorder which, in the opinion of the investigator, may put the participants at risk because of participation in the study, may influence the result of the study, or affect the participant's ability to participate in the study.