The purpose of this study is to determine if the phase of the menstrual cycle (opposed versus unopposed estrogen phase) affects eating behavior, including types of foods preferred, wanting/desire for specific foods, and perception of hunger.
This is an observational study to investigate the effect of endogenous estrogen (E2) and progesterone (P4) levels on eating behavior and energy-yielding nutrient metabolism. Eating behavior will be assessed during a complete menstrual cycle during the first month of the study. Participants will track the follicular and luteal phases of their menstrual cycle using urinary leuteinizing hormone (LH) kits, they will record information about their food intake, eating behaviors, and appetite in their home environments, and they will complete two laboratory test days: one test day scheduled during their follicular phase, and one test day scheduled during their luteal phase.
Study Type
OBSERVATIONAL
Enrollment
24
USDA, ARS, Western Human Nutrition Research Center
Davis, California, United States
Eating behavior
Eating behavior, including types of foods preferred, wanting/desire for specific foods, and perception of hunger will be measured using four questionnaires; Eating inventory: to assess dietary restraint, Stanford Brief Physical Activity Questionnaire, Food craving questionnaire, Eating behavior and attitudes questionnaire
Time frame: At follicular (day 11) and luteal (day 26) phase of menstrual cycle
Insulin sensitivity
To determine if the phase of the menstrual cycle affects insulin sensitivity, we will measure plasma insulin, glucose and non-esterified fatty acids (NEFA).
Time frame: At follicular (day 11) and luteal (day 26) phase of menstrual cycle
Serum lipid profile changes
Blood samples will be analyzed for lipids and lipoproteins, including apoproteins A1, B, C-II, C-III, and E.
Time frame: At follicular (day 11) and luteal (day 26) phase of menstrual cycle
Urinary c-peptide
The level of urinary C-peptide and creatinine will be measured in a 24-hour urine collection.
Time frame: At follicular (day 11) and luteal (day 26) phase of menstrual cycle
Estradiol
Estradiol measured in serum in relation to appetite variables
Time frame: At follicular (day 11) and luteal (day 26) phase of menstrual cycle
progesterone
Progesterone measured in serum in relation to appetite variables
Time frame: At follicular (day 11) and luteal (day 26) phase of menstrual cycle
Dehydroepiandrosterone (DHEA)
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Dehydroepiandrosterone measured in serum in relation to appetite variables
Time frame: At follicular (day 11) and luteal (day 26) phase of menstrual cycle
Sex Hormone Binding Globulin (SHBG)
SHBG measured in serum in relation to appetite variables
Time frame: At follicular (day 11) and luteal (day 26) phase of menstrual cycle
Endocannabinoid profile
Endocannabinoid profile measured in serum to assess appetite
Time frame: At follicular (day 11) and luteal (day 26) phase of menstrual cycle
Leptin
Leptin measured in serum to assess appetite
Time frame: At follicular (day 11) and luteal (day 26) phase of menstrual cycle