Kinetics of IgE Memory B Cells, Plasmablasts and Plasma Cells After Whole Lung Allergen Challenge in Mild Asthmatics

Inclusion Criteria: * Male and female volunteers 18 through 65 years of age. * Females must not be actively seeking pregnancy, must be using adequate and effective contraception * General good health * Mild to moderate, stable, allergic asthma * History of episodic wheeze and shortness of breath; FEV1 at baseline at least 70% of the predicted value * Able to understand and give written informed consent and has signed a written informed consent form approved by the investigator's REB * Positive methacholine challenge * Positive skin-prick test to common aeroallergens (including cat, dust mite, grass, pollen) * Positive allergen-induced airway bronchoconstriction (a fall in FEV1 of at least 20% from baseline) Exclusion Criteria: * A worsening of asthma or a respiratory tract infection within 6 weeks preceding study entry * History of clinically significant hypotensive episodes or symptoms of fainting, dizziness, or lightheadedness * History or symptoms of clinically significant autoimmune disease * History of clinically significant hematologic abnormality, including coagulopathy * Be pregnant or lactating * Use of corticosteroids, immunosuppressives, anticoagulants (warfarin or heparin) within 28 days prior to randomization into the study * Use of nonsteroidal anti-inflammatory drugs (NSAIDs) within 48 hours of dosing or aspirin with 7 days of dosing * Have chronic use of any other medication for treatment of allergic lung disease other than short- and intermediate-acting ß2-agonists or ipratropium bromide * Use of caffeine-containing products or medications for 12 hours or alcohol or over the counter drugs including aspirin, cold and allergy medications for 48 hours or inhaled bronchodilators for 8 hours prior to methacholine and allergen challenges * Use of tobacco products of any kind currently or within the previous 12 months, or smoking history \> 10 pack years. * Lung disease other than mild to moderate allergic asthma * Unwillingness or inability to comply with the study protocol for any other reason.