Metabolomic Embryo Profiles of Obese in Vitro Fertilization (IVF) Patients and Their Relationship With Polycystic Ovary Syndrome (PCO)

Instituto Valenciano de Infertilidad, IVI VALENCIA28 enrolled

Overview

The present study aims to elucidate if there is a metabolomic profile alteration in the embryos of obese women in order to understand if the reduced implantation rate observed in these patients is directly related to this factor. Furthermore, the investigators seek to establish if there is any difference between obese women with Polycystic Ovary Syndrome (PCO) and without PCO. The investigators compare these metabolomic profile embryos with embryos of egg-donation programme.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Infertility Obesity

Interventions

Culture medium metabolomic analysisOTHER

During the embryo transfer at day 3, the investigators keep and freeze a small drop of the culture medium of each embryo transferred. Each drop of culture medium will have a volume of 40-50 microliters, before freezing is spun at 10.000 rps to avoid any possible contamination. The frozen media culture is analyzed for the metabolomic profile.

Eligibility

Sex: ALLMin age: 18 YearsMax age: 38 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * BMI \> 30 kg/m2 * Age \< 38 years old * Non smokers * First IVF cycle (groups 2 \& 3, not Control group) * Normal uterus (seen by ultrasound) * normal basal hormonal profile(FSH,LH,estradiol,testosterone,androstenedione, insulin, SHBG, glucose, AMH) * Antral follicle count(\> 5 FA for each ovary) * Menses every 25-35 days. Exclusion Criteria: * \< 5 or \> 20 oocytes retrieved after stimulation cycle * estradiol \>3000 pg/ml or progesterone \> 1,5 ng/ml on the hCG day. * \< 2 evolutive embryos in the culture medium.

Outcomes

Primary Outcomes

Metabolic profile of culture media of Day 3 embryos

Determine differences in the metabolomic profile of the culture media of day 3 embryos, obtained after in vitro fertilization, according to the presence of obesity associated or not with polycystic ovary syndrome.

Time frame: 3 days